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基因编辑技术及其在畜牧业中的应用研究进展

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基因编辑技术为畜牧业进行新型分子育种开创了新天地,传统的转基因技术由于整合位点不可控、遗传不稳定等因素而难以在畜牧业发挥作用.胚胎干细胞(ES细胞)和同源重组(HR)相结合可以做到定点整合、稳定遗传,但因为畜禽干细胞难以获得而无法推广.以锌指核酸酶(ZFN)、TALEN和CRISPR/Cas9为代表的新型基因编辑技术结合体细胞核移植(SCNT)以及显微注射技术,为畜禽遗传改良开辟了新思路.本综述了ES结合HR的传统基因编辑技术和ZFN、转录激活因子样效应物核酸酶(TALEN)和成簇有规律间隔的短回文重复序列(CRISPR)以及其相关蛋白(Cas9,Cpf1)的研究及其在畜禽基因编辑中的应用进展.
Developments and Application of Gene Editing Technology in Animal Husbandry
Classic transgenic technology couldn't play its great role in animal breeding because of randomly integration and unstable heredity of exogenous genes.A new world has been opened for molecular breeding of animals by gene editing.Embryonic stem (ES) cells and homological recombination (HR) could make targeted integration and stable heredity of introduced genes,but this technique was difficult to apply owing to unavailable ES cells in farm animals.New path was developed for molecular breeding in the farm animals by the new gene editing technologies including the zinc finger nuclease (ZFN),the transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9) combine with somatic cell nuclear transfer (SCNT) and/or pronuclear microinjection.The progress and applications of ES cells combined with HR,ZFN,TALEN,CRISPR and related proteins (Cas9,Cpf1) in farm animals were reviewed in this study.

Gene editingHRZinc finger nucleaseTALENCRISPR/Cas9CRISPR/Cpf1

李莉、毕延震、华再东、任红艳、肖红卫、刘西梅、郑新民

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湖北省农业科学院畜牧兽医研究所,动物胚胎工程及分子育种湖北省重点实验室,武汉,430064

基因编辑技术 同源重组 锌指核酸酶 TALEN CRISPR/Cas9 CRISPR/Cpf1

本研究由转基因生物新品种培育重大专项本研究由转基因生物新品种培育重大专项本研究由转基因生物新品种培育重大专项湖北省农业科技创新中心湖北省科技支撑计划湖北省农科院青年基金

2016ZX08006001-0052016ZX08006002-0062016ZX08010003-0062016-620-000-001-0272014BBB0102016NKYJJ19

2016

10.13417/j.gab.035.003410

基因组学与应用生物学
广西大学

基因组学与应用生物学

CSTPCDCSCD北大核心
影响因子：1.108
ISSN：1674-568X
年,卷(期)：2016.35(12)
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