Cloning and Expression Analysis of PmCYP77A1 Gene in Pinus massoniana
A eDNA region encoding CYP77A1 was found in the transcriptome sequencing analysis of insect-resistant materials in Pinus massoniana,and it was named PmCYP77A1.The result of sequence analysis showed that the gene was 1 620 bp in total length,encoding 539 amino acids.Amino acid sequence analysis showed that there was a conservative domain of CYP450 gene family in this gene,but the last base in FXXGXXXCXG conserved sequence of CYP77A1 gene was replaced by base A.The nucleic acid sequence coverage and homology of PmCYP77A1 was poorer when compared with those of some other plants,but the amino acid sequence alignment coverage reached 88％～96％,while its homology was low,generally between 50％～55％.In genetic clustering,PmCYP77A1 was closest to that of white spruce,but it was far with other angiosperms.Expression analysis by fluorescent quantitative PCR technique showed that PmCYP77A1 expressed in all tissues of Pinus massoniana,which had the highest expression level in tender sterms,male and female flowers,and tender leaves,and the low-est in cones,mature tissues and roots.In different insect-resistant materials,daily variation of PmCYP77A1 expression appeared the trend of first rose and then declined,and the expression of this gene started early in insect resistance materials.This study laid a foundation for further analysis of the biological function of PmCYP77A1 and its role in insect-resistant defense in Pinus massoniana.
NETL
NSTL
万方数据
维普
