Bioinformatics Analysis of MADS-box Gene Family in Pineapples
MADS-box transcription factors are vital regulators participating in plant growth and development of plants.In order to understand the sequences characteristics and classification of pineapple MADS-box transcription factors,we carried out sequential analysis of MADS-box of the whole genome,including the number of MADS-box transcription factors,constitution of exons and introns,the basic characteristics of coding proteins,and phylogenetic analysis.The results showed that 40 MADS-box transcription factors in pineapples were identified.Gene structure analysis found that 8 MADS-box genes had 6 introns and 7 extrons,which correspond with the typical structure of plant MADS-box gene.At the same time,27 pineapple MADS-box transcription factors coded 200～300 amino acid,among which AcMADS40 was the smallest one,encoding 104 amino acid,and AcMADS08 was the largest one,encoding 436 amino acid.There were 31 alkalescent proteins,5 neuter proteins and 4 acidic proteins in the 40 MADS-box proteins.The secondary structure analysis showed that 6 MADS-box proteins had the highest ratio of random coils,and AcMADS02 had 25 random coils,Alpha helixes and 5 β-turns.The other 33 MADS-box proteins had the highest ratio of Alpha helixes.Subcellular localization analysis indicated that most MADS-box proteins were located in nucleus,while others were scattered in other cell organs.Conservative motif-searching analysis showed that more than 85％ of MADS-box had motif 1 and motif 2,which indicated that motif 1 and motif 2 were important conserved motifs in pineapple MADS-box proteins.Phylogenetic analysis found that 26 pineapple MADS-box genes belonged to in type Ⅱ (MIKC),while 14 belonged to type Ⅰ.Futhermore,it could be classified into 11 subgroups,and there were MADS-box genes in these subgrouops FLC and AGL15.
NETL
NSTL
万方数据
维普
