Effects of Momordica charantia Saponin and Sodium Nitrate on Rumen Bacterial Diversity of Goats
Rumen bacteria plays an important role in ruminant rumen fermentation.The present study was conducted to investigate the effects of Momordica charantia saponin and sodium nitrate on rumen bacterial diversity in vitro fermentation by using terminal restriction fragment length polymorphism and clone library technologies.The results showed that the Momordica charantia saponin significantly influenced bacterial richness indexes and shannon-weiner index at 6 h,24 h of in vitro incubation.Meanwhile,the saponin had a greater influence on the rumen bacterium R-7 and Butyrivibrio bacteria.No significant effect of sodium nitrate on bacterial diversity indexes was observed.However,the sodium nitrate addition influenced the bacterial community to a certain extent,and promoted the growth ofRuminobacter albus at 24 h of incubation.