The Optimization of Extracting Xylan from Sugarcane Bagasses by Using Potassium Hydroxide and H2O2 and the Analysis of Xylooligosaccharides Produced by Xylanase from Sugarcane Bagasse
Sugarcane bagasse (SB) contains approximately 40％ cellulose and 20％ hemicellulose,which is a kind of ideal feedstock for biorefinery to produce the high value-added biochemical products.In this study,response surface methodology was employed to optimize the parameters of xylan which were extracted by potassium hydroxide (KOH) and H2O2,including KOH concentration,H2O2 concentration and treating temperature.Taking the extraction rate of SB xylan as index,the optimized conditions were 10.0％ KOH,2.36％ H2O2,50℃,6 hours,bringing that the extraction rate of SB xylan was 78％.The xylo-oligosaccharude,mainly including xylobiose and xylotriose,produced by the sugarcane bagasses xylan which was extracted by the hydrolysis of recombinant endo-xylanase,was hydrotyzed for 24 hours under the conditions of 36 g/L of substrate concentration,36℃ reaction temperature,pH 5.0 and 800 U/g substrate ofxylanase,and its conversion was 43％.
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维普
