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蒙古沙冬青基因组调查及SSR分析

Genome Survey and SSR Analysis of Ammopiptanthus mongolicus

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蒙古沙冬青是第三纪冰川孑遗物种,具有极强的耐逆性,是研究植物耐逆分子机制的良好材料.利用高通量测序技术和生物信息学工具预测蒙古沙冬青的基因组大小及杂合度,进行SSR分子遗传标记的初步鉴定.蒙古沙冬青基因组大小约为812Mb,杂合率为0.506%,基因组杂合度较高.对基因组进行拼接,进行SSR分子遗传标记分析,共鉴定183 102个SSR,不同类型核苷酸重复差异较大,其中,二核苷酸重复中的AT/TA含量最高,四核苷酸重复最少,共占总数的1.48%.
Ammopiptanthus mongolicus is a relict species of tertiary glacier.It has strong resistance to stress,and is a good material for the study of the molecular mechanism of plant resistance.The genome size and heterozygosity were predicted by high throughput sequencing and bioinformatics tools,which were used for the preliminary identification of simple sequence repeat (SSR) ofAmmopiptanthus mongolicus.The genome size of Ammopiptanthus mongolicus was about 812 Mb,the heterozygosity was 0.506%,and the genome heterozygosity was high.Genome sequencing was assembled and subjected to SSR.A total of 183 102 SSRs were obtained and different types ofnucleotides repeated differently.Among them,the content of AT/TA in dinucleotide repeats was the highest,and four nucleotides repeated least,accounting for 1.48% of the total.

Ammopiptanthus mongolicusGenome sizeHeterozygositySSR

周佳熠、王雪、高飞、周宜君

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中央民族大学生命与环境科学学院,北京,100081

蒙古沙冬青 基因组大小 杂合度 SSR

国家自然科学基金国家自然科学基金国家大学生创新训练计划国家大学生创新训练计划

3137035631670335GCCX2016110019SHSY20161-20022

2017

基因组学与应用生物学
广西大学

基因组学与应用生物学

CSTPCDCSCD北大核心
影响因子:1.108
ISSN:1674-568X
年,卷(期):2017.36(10)
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