Cloning and Bioinformatics Analysis of Brassinosteroid insensitive 1 Gene in Eucommia ulmoides
To analyze the function of Brassinosteroid relative genes in Eucommia ulmoides. The homologous sequence of BRI1 was found according to the annotation of Eucommia ulmoides transcriptase database constructed by our laboratory. The reference sequence of the gene was found by comparison with genome data. Specific primers were designed, and the 3 612 bp DNA sequence was obtained, which named EuBRI1. Sequence analysis revealed that EuBRI1 is a complete open reading frame without introns, encoding a protein of 1 203 amino acids. The EuBRI1 encoding protein has isoelect point (PI) of 6.31 and a calculated molecular weight about 131.361 kD, with transmembrane regions and signal peptitde has a Kinase conserved domain of BRI1 family. In the secondary structure, the percentage of alpha helix, β-extended, and random coil were 31.50％, 55.94％, 12.55％, respectively. The phylogenetic tree analysis indicates the level evelution of EuBRI1 is similarity with Solanum pimpinellifolium BRI1. We acquired valuable information which can be used to study the biological function of the EuBRI1 gene.
NETL
NSTL
万方数据
维普
