Genome-wide Identification and Expression Analysis of DREB Gene Family in Pear
To id entify the DREB gene of pear, this research analyzed its physiological characteristics and structure, and studied the interaction of DREB protein and expression difference under different stress. The DREB transcription factor of pear was identified and analyzed by bioinformatics method. For instance, local BLAST, Pfam, etc. were used to search and identify DREB protein; Protparam, Signal P, SPOMA, MEME database, DNAMAN, MEGA6.0, etc. were used to analyze structural characteristics and evolutionary relationship of protein; functional interaction analysis of DREB protein was carried out based on the String database; meanwhile, homologous expression analysis was carried out by using Arabidopsis thaliana Illumia RNA-Seq data. 60 DREB genes were identified from the whole genome of pear, and were divided into 6 subgroups (A1～A6) by cluster analysis with Arabidopsis thaliana DREB gene. The number of encoded amino acid residues was between 99 and 496, with an average of 250 amino acid residues, and the whole was weakly acidic. The conserved motif and gene structure of the Pbr DREBs protein were systematically analyzed. It was found that these Pbr DREBs proteins contained a typical AP2/ERF domain, which contained about 55 amino acid residues, most of them started with YRG conserved elements, and terminated with RAYD conserved elements. The DREB transcription factor played an important role in the growth and development of pear and signal transduction of its various stress responses, and there might be interactions between the members of the subgroups in adversity regulation, which had been studied systematically by the functional linkage network between Pbr DREBs proteins. Among them, CBF transcription factor (A1 subgroup) played an important role in mutual regulation of protein. And the homologous analysis of Pbr DREBs protein was carried out under abiotic stresses such as low temperature and drought through homologous Arabidopsis thaliana RNA-Seq data. It showed that DREB protein played an important role in various stresses. The preliminary identified 60 DREB genes of pear were similar to DREB genes in Arabidopsis thaliana and other reported species in terms of function, structure and characteristics, which played an important role in adversity such as low temperature and drought.
NETL
NSTL
万方数据
维普
