Some timber from Phoebe and Machilus are very precious. However, the wood anatomical structure features of some species of Phoebe and Machilus are very similar which made it difficult to accurately identify the wood of two genera based on the traditional identification method. By using the DNA barcode technology, five kinds of timber from Phoebe and Machilus were tried to be identified at molecular level. Five species (Phoebe bournei, Phoebe zhennan, Phoebe sheareri, Machilus oreophila, Machilus pauhoi) were choosen as research object and wood from five tree species were collected as experimental materials. DNeasy ? Plant Mini Kit (QIAGEN) was adopted to extract the timber DNA. Four DNA section of ycf3, psb C-trn S, rpo C1 and ITS from five species were amplified by PCR, and PCR product were sequenced and aligned. The results showed that there are six polymorphism sites on ycf3 sequence between five species. Based on the difference, Phoebe zhennan, Machilus oreophila and Machilus pauhoi could be distinguished while the other two could not be. There are seven polymorphism sites on psb C-trn S sequence. Based on the difference, Phoebe sheareri, Machilus oreophila and Machilus pauhoi could be identified. There is only one polymorphism site on the ITS sequence which could distinguish just between two different genera but not species. The rpo C1 sequences of the five tree species are exactly the same and could not be used to distinguish between these species. However, the combination of ycf3 and psb C-trn S DNA barcodes can distinguish the five wood species. From the phylogenetic tree obtained by the NJ method based on the ycf3 sequence, five species can be correctly clustered as a reference for distinguishing these tree species.
NETL
NSTL
万方数据
维普
