本研究利用分子标记技术,分析所收集的澳洲坚果(Macadamia spp.)种质的遗传多样性,构建其指纹图谱,旨在为澳洲坚果遗传资源保护、良种选育和品种鉴定提供技术保障.基于转录组测序结果,开发出了 12个特异性强、多态性高的表达序列标签-简单重复序列(expressed sequence tags-simple sequence repeat,EST-SSR)分子标记.对51份澳洲坚果种质进行SSR荧光标记毛细管电泳检测,分析种质的遗传多样性,进行系统聚类及指纹图谱构建.结果表明,12对EST-SSR分子标记引物共检测到72个等位基因,平均每对引物扩增出6个等位基因,多态性信息含量(polymorphic information content,PIC)平均值为0.632,标记多态性高.在遗传相似系数为0.422时,可以将51份澳洲坚果种质分成6类;采用引物代码和多态性片段长度组合法构建了 51份澳洲坚果种质的分子指纹图谱.本研究新开发的12个EST-SSR分子标记,可用于澳洲坚果种质资源的亲缘关系分析和品种鉴定.
Molecular Fingerprint Construction of Macadamia spp.Based on EST-SSR Mo-lecular Markers
In this study,the genetic diversity of Macadamia spp.germplasm collected was analyzed by molecular marker technology,and construct the fingerprint,so as to provide technical support for the protection of macadamia genetic resources research,breeding of improved varieties and variety identification of macadamia.Based on the results of transcriptomic sequencing,12 expressed sequence tags-simple sequence repeat(EST-SSR)molecular markers with strong specificity and high polymorphism were deve-loped.51 maca-damia germplasm samples were detected by capillary electrophoresis with SSR fluorescent markers to analyze the genetic diversity of germplasm,perform systematic clustering and construct fingerprint.The results showed that a total of 72 alleles were detected by 12 pairs of EST-SSR primers,and an average of 6 alleles were amplified from each primers.The average polymorphic information content(PIC)was 0.632,indicating high polymorphism of these EST-SSR markers.When the genetic similarity coefficient was 0.422,51 macadamia germplasms could be divided into 6 categories.The molecular fingerprints of 51 macadamia germplasms were constructed by using primer code and polymorphism fragment length.In this study,12 EST-SSR markers were developed,which could be used for ge-netic relationship analysis and variety identification of macadamia germplasm resources.
NETL
NSTL
万方数据
维普
