Pyramided Breeding by Use of Nilaparvatal lugens Resistance Genes Bph3 and Bph24(t)in Rice
The rice(Oryza sativa)production is affected by many diseases and pests,such as Nilaparvatal lugens.Cultivating resistant cultivars through polymerizing different resistance genes is the most effective strategies to cope with these biotic stresses.Traditional aromatic rice cannot be popularized within a large area due to the deficiency of resistance.In this study,a series of maintainer line and restorer line of high quality three-line hybrid rice,which containing rice blast resistance gene(Pi2,Pib or Pimh),bacterial blight re-sistance gene(Xa23)or aroma gene(badh2)were hybridized with donor parents containing N.lugens resistance gene Bph3 or Bph24(t).As a result,the N.lugens resistance genes were introduced into elite breeding lines through marker-assisted selection(MAS).A total of 41 stable pyramided lines containing N.lugens resistance genes,and aggregated with other target genes in different combina-tions were screened.The identification of resistance,aroma and agronomic traits showed that each pyramided line not only possesses a high level of N.lugens resistance compared to the recipient parents,but also exhibits satisfactory agronomic traits,including dual-re-sistance,triple-resistance and/or aroma.It can be used as germplasm resources for multi resistance and high-quality breeding of rice.