首页|花鲈igf3基因SNP位点的鉴定及其与生长性状的关联分析

花鲈igf3基因SNP位点的鉴定及其与生长性状的关联分析

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  • 维普
花鲈(Lateolabrax maculatus)是我国最重要的海水经济鱼类之一,近年来其年产量已突破19万吨,是构成我国"菜篮子"工程的重要组成部分.但是,由于缺乏有效的遗传标记导致花鲈良种选育技术不成熟,这成为限制我国花鲈产业健康发展的主要瓶颈之一.为筛选花鲈胰岛素样生长因子3(insulin-like growth factor 3,igf3)基因的单核苷酸多态性(single nucleo-tide polymorphism,SNP)位点以及了解其与生长性状的关联性,本研究对同批次9月龄花鲈群体开展igf3基因SNP位点筛选及其与生长性状的关联性分析.实验结果显示,花鲈igf3基因上存在4个SNP位点,分别位于起始密码子下游C+2 990T、G+3 037A、C+3 303G、G+3 602A处,其中前3个位点位于内含子区,第4个位点位于外显子区.尽管单个SNP位点与花鲈生长性状之间并无显著关联性(P＞0.05),但双倍型与花鲈生长性状之间却表现出明显的关联性(P＜0.05).其中,双倍型D2(CCGAGGC)花鲈个体各生长性状表现最优,其体重、头长、体高和体宽均显著高于D3型(CTGGCGCC)个体的(P＜0.05).本研究为筛选具备生长优势的花鲈提供了候选基因,有利于花鲈育种工作的开展.
SNP Detection of igf3 Gene and Its Association with Growth Traits in Lateo-labrax maculatus
Spotted seabass(Lateolabrax maculatus)is one of the most important seawater economic fish in China,and its annual out-put has exceeded 190 000 tons in recent years,which is an important part of the"vegetable basket"project of our daily life.However,due to the lack of effective genetic markers,the breeding technology of spotted seabass is limited,which has become one of the bottle-necks restricting the healthy development of spotted seabass industry in China.In the present study,to explore the association of the single nucleotide polymorphism(SNP)of insulin-like growth factor 3(igf3)gene and the growth traits in spotted seabass,the SNPs characteristics of igf3 gene in spotted seabass population were detected.The results showed that there were four SNP sites on igf3 gene,located at C+2 990T,G+3 037A,C+3 303G,and G+3 602A downstream of the start codon,respectively.The first three SNPs were located in the intron region,and the fourth one was located in the exon region.Although there was no significant correlation between in-dividual SNP and growth traits of spotted seabass(P＞0.05),the diploids showed significant correlation with grow traits of spotted sea-bass(P＜0.05).Among them,the doubled haplotype D2(CCGAGGC)populations showed the greater presentation in four detected growth traits(body weight,head length,body height and body width),especially significantly higher than that of D3(CTGGCGCC)populations(P＜0.05).This study provids candidate genes for screening spotted seabass with potential growth advantage,which is be-nificial for the development of spotted seabass breeding work.

Lateolabrax maculatusigf3Single nucleotide polymorphism(SNP)Growth traits

陶欣、邱丽华、张博、王鹏飞、闫路路、赵超、林云想、林锋

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大连海洋大学水产与生命学院,大连,116000

中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室,广州,510300

中国水产科学研究院,农业农村部水生动物基因组学重点实验室,北京,100141

饶平县西海岸生物科技有限公司,潮州,515725

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花鲈 igf3 单核苷酸多态性(SNP) 生长性状

广东省重点领域研发计划项目国家重点研发计划项目中国水产科学研究院中央级公益性科研院所基本科研业务费专项中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项广东省农业农村厅乡村振兴战略专项

2021B02020200022022YFD24005032020TD212021SD112022-SPY-00-009

2024

10.13417/j.gab.043.000109

基因组学与应用生物学
广西大学

基因组学与应用生物学

CSTPCD北大核心
影响因子：1.108
ISSN：1674-568X
年,卷(期)：2024.43(1)
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