Construction of a GUS Reporter Vector for Riboswitch Screening and Identifica-tion in Xanthomonas
Riboswitch is a regulatory RNA element located in the 5'-untranslated region(5'UTR)of a messenger RNA(mRNA),it regulates the expression of the mRNA by directly binding to a specific metabolite molecule.Riboswitches are widely distributed in bac-teria and have been shown to play an important role in various physiological processes including the primary metabolism and pathogene-sis.Although a large number of riboswitches have been identified in model bacteria such as Escherichia coli,only one riboswitch was found in the important plant pathogenic bacterial genus Xanthomonas,majorly due to lacking a simple and highly efficient method for ri-boswitch screening and identification in Xanthomonas.For a high throughput and genome-wide riboswitch screening and identification in Xanthomonas,in this work,a GUS reporter vector,pL3-SD+gusA,was designed and constructed.This vector is suitable for the validation of riboswitch candidates in Xanthomonas,it also can achieve a high throughput riboswitch screening and identification in Xanthomonas through construction of a genomic DNA expression library.
XanthomonasGUS reporter vectorRiboswitchHigh throughput screening and identification
NETL
NSTL
万方数据
维普
