Transcriptome Analysis of Tibetan Medicine Phlomoides rotata at Different Alti-tudes in Qinghai-Tibet Plateau
In order to study the gene expression difference and response mechanism of Phlomoides rotata in different altitude habitats,differentially expressed genes(DEGs)annotation and functional enrichment analysis were performed based on NR,GO and KEGG da-tabases in this study,to obtain the DEGs and associated metabolic pathways of P.rotata,and to analyze the phylogenetic of the medic-inal genes obtained by annotation.The results showed that the DEGs of P.rotata at different altitudes were mainly enriched in plant hormone signal transduction,amino acid biosynthesis,terpenoids and other secondary metabolism biosynthesis pathways.Of those,four genes play a key role in response to cold-stress.Three P5βR genes were identified,two of which encode key enzymes in the iridoid biosynthesis pathway.Phylogenetic analysis showed that the P5βR gene subfamily was clustered into two branches(P5βR cluster I and P5βR cluster Ⅱ).In this study,we identified that P.rotata adapts to the high altitude habitat of the Qinghai-Tibet Plateau through the participation of multiple metabolic pathways and the regulated expression of key genes.Our study insights into the adapta-tion mechanism of P.rotata to different altitudes and cold tolerance breeding of P.rotata,lays a foundation for the study of P5βR gene subfamily and iridoid biosynthesis.
NETL
NSTL
万方数据
