Research Progress in Mining,Mutation and Application of Glucose-tolerant β-glucosidase Genes
Reducing enzyme cost used in lignocellulosic biorefineries is important to promote the green utilization of lignocellulose.β-glucosidase,a rate-limiting enzyme during enzymatic hydrolysis of cellulose,is inhibited by a high concentration of glucose.Mining and mutating the genes of glucose-tolerant β-glucosidases are important strategies.This paper summarizes the progress made in recent years in mining glucose-tolerant β-glucosidase genes,analyzing the molecular mechanism of enzyme tolerance to glucose,mutation of β-glucosidase gene to improve the glucose-tolerance,and genetic engineering of modified microbial strains based on β-glucosidase genes,and then perspectives.This paper will provide references for constructing genetically engineered strains with a high yield of glu-cose-tolerant β-glucosidase,which is of great significance to the development of lignocellulosic biorefineries.
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