耐糖β-葡萄糖苷酶基因的挖掘、突变及应用研究进展
Research Progress in Mining,Mutation and Application of Glucose-tolerant β-glucosidase Genes
张婷 1宁远妮 2冯家勋 2赵帅2
作者信息
- 1. 南宁学院食品与质量工程学院,南宁,530200
- 2. 广西大学生命科学与技术学院,南宁,530004
- 折叠
摘要
降低木质纤维素生物炼制的用酶成本,对推动木质纤维素的绿色利用至关重要.作为纤维素水解过程中的限速酶,β-葡萄糖苷酶酶解活性受高浓度葡萄糖的胁迫抑制.目前,挖掘和突变耐糖β-葡萄糖苷酶的基因是解决这一问题的重要策略.本综述总结了近年来在耐糖β-葡萄糖苷酶基因的挖掘、酶耐糖分子机制的分析、旨在提高酶耐糖性能的酶基因突变以及基于β-葡萄糖苷酶基因的微生物菌株基因工程改造等方面所取得的进展,并进行了展望.本综述为构建高产耐糖β-葡萄糖苷酶的基因工程菌株提供参考,这对推动木质纤维素生物炼制工业化进程具有重要意义.
Abstract
Reducing enzyme cost used in lignocellulosic biorefineries is important to promote the green utilization of lignocellulose.β-glucosidase,a rate-limiting enzyme during enzymatic hydrolysis of cellulose,is inhibited by a high concentration of glucose.Mining and mutating the genes of glucose-tolerant β-glucosidases are important strategies.This paper summarizes the progress made in recent years in mining glucose-tolerant β-glucosidase genes,analyzing the molecular mechanism of enzyme tolerance to glucose,mutation of β-glucosidase gene to improve the glucose-tolerance,and genetic engineering of modified microbial strains based on β-glucosidase genes,and then perspectives.This paper will provide references for constructing genetically engineered strains with a high yield of glu-cose-tolerant β-glucosidase,which is of great significance to the development of lignocellulosic biorefineries.
关键词
生物炼制/β-葡萄糖苷酶/葡萄糖胁迫/工程菌Key words
Biorefineries/β-glucosidase/Glucose stress/Engineered strains引用本文复制引用
基金项目
广西壮族自治区高等学校中青年教师科研基础能力提升项目(2023)(2023KY1871)
南宁市"邕江计划"青年人才专项(2023)(RC20230202)
出版年
2024
NETL
NSTL
万方数据