苦荞(Fagopyrum tataricum)RILs群体果壳率相关性状的变异分析和QTL定位
Variation Analysis and QTL Mapping of Grain Shell Rate-related Traits of RILs Population in Tartary Buckwheat(Fagopyrum tataricum)
薛贤滨 1黎瑞源 2任蓉蓉 1贾琼 1陈峥峰 1陈庆富 1王莉花 3石桃雄1
作者信息
- 1. 贵州师范大学生命科学学院贵州省荞麦工程技术研究中心,贵阳,550001
- 2. 贵州师范大学贵州省信息与计算科学重点实验室,贵阳,550001
- 3. 云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,昆明,650205
- 折叠
摘要
为选育薄果壳大粒的苦荞(Fagopyrum tataricum)品种和挖掘果壳率相关性状候选基因,本研究以'小米荞'(米荞型)和'晋荞麦2号'(非米荞型)为亲本构建的重组自交系(recombinant inbred lines,RILs)群体为供试材料,分析了两个生长季节苦荞RILs群体的果壳率、百粒重、百粒果仁重和百粒果壳重等4个性状的遗传变异和数量性状座位(quantitative trait lo-cus,QTL).结果显示,非米荞型亲本和非米荞型株系的4个果壳率相关性状均值均高于米荞型亲本和米荞型株系的.米荞型和非米荞型株系的4个果壳率相关性状变异系数分别在9.01％～15.66％和8.21％～14.04％.果壳率与百粒重、百粒果仁重和百粒果壳重均呈极显著正相关.通过聚类分析筛选到米荞型株系R160、R198、R199和R46,以及非米荞型株系R102、R120、R125、R142、R157和R178在两个生长季节均表现低果壳率和高百粒重的特点,可作为薄壳大粒苦荞品种选育的候选材料.两个生长季节共检测出17个QTL,其中百粒重、百粒果仁重、百粒果壳重和果壳率的QTL数目分别为5个、4个、5个和3个,单个QTL的表型贡献率为3.76％～48.65％.QTL簇qC1.2含果壳率、百粒重、百粒果仁重和百粒果壳重稳定主效QTL各1个,与已经报道的控制果壳类型和粒重的主效QTL重叠.研究结果可为苦荞果壳率相关性状的分子改良、基因的精细定位和克隆提供理论依据.
Abstract
In order to breed the tartary buckwheat(Fagopyrum tataricum)varieties with thin-shell and large-grain and excavate the candidate genes for the grain shell rate-related traits,this research analyzed the genetic variation and quantitative trait locus(QTL)for grain shell rate,hundred grain weight,hundred kernel weight and hundred grain shell weight of the recombinant inbred lines(RILs)population derived from the cross of'Xiaomiqiao'(rice type)and'Jinqiaomai 2'(non-rice type)under two growing seasons.The four grain shell rate-related traits of non-rice type parent and lines were significantly higher than those of rice type parents and lines.The coefficient of variation of the four grain shell rate-related traits in rice type lines and non-rice type lines ranged from 9.01％to 15.66％and 8.21％to 14.04％,respectively.The grain shell rate had an extremely significant positive correlation with hundred grain weight,hundred kernel weight and hundred grain shell weight.Cluster analysis showed that rice type lines R160,R198,R199 and R46 and non-rice type lines R102,R120,R125,R142,R157 and R178 with lower grain shell rate and higher hundred grain weight under the two growing seasons,which could be used as candidate materials for breeding tartary buckwheat varieties with thin-shell and large-grain.A total of 17 QTL were detected under two growing seasons,including 5 for hundred grain weight,4 for hundred kernel weight,5 for hundred grain shell weight and 3 for grain shell rate,explaining 3.76％～48.65％of the phenotypic variance.The QTL cluster qC1.2 contained each one stable major QTL for the four tested traits,which overlapped with the reported stable major QTL for shell type and hundred grain weight.These results provide a theoretical basis for fine mapping,cloning of genes and molecular improvement of grain shell rate-related traits in tartary buckwheat.
关键词
苦荞/果壳率/遗传变异/QTL定位/候选基因Key words
Tartary buckwheat/Grain shell rate/Genetic variation/QTL mapping/Candidate gene引用本文复制引用
基金项目
国家自然科学基金(32260489)
国家现代农业产业体系荞麦育种岗位科学家专项(CARS-07-A5)
云南省科技重大专项与重点研发计划(202202AE090020)
贵州师范大学学术新苗培养及创新探索专项(2017)(黔科合平台人才[2017]5726-18)
出版年
2024
NETL
NSTL
万方数据