马铃薯GRF基因家族的鉴定和表达模式分析
Genome-wide Identification and Expression Analysis of the GRF Gene Family in Potato
石蒙蒙 1王德颖 1谢浩杰 1秦志广 1刘紫婕 1韩宝佳 1呼雨君 1郭文慧 1孙永旺1
作者信息
- 1. 聊城大学农学与农业工程学院,聊城,252100
- 折叠
摘要
生长调节因子(growth-regulating factor,GRF)家族基因编码植物特有的转录因子,在植物生长发育和胁迫响应等许多生物学过程中发挥重要调节作用.本研究利用生物信息学方法全面鉴定了马铃薯(Solanum tuberosum)GRF基因家族,并分析了其表达模式.在马铃薯基因组中共发现了12个GRF家族基因(StGRF1～StGRF12),其编码的蛋白均含有QLQ结构域和WRC结构域;StGRF基因分布在马铃薯的9条染色体上,染色体片段复制可能导致了该家族基因在马铃薯基因组中的扩张;系统进化分析显示,StGRF可以分为5组,同组的基因成员具有相似的基因结构,且其编码的蛋白具有相似的保守结构域分布;StGRF成员启动子区域存在丰富的激素和胁迫响应相关元件;表达模式分析显示,StGRF在马铃薯不同发育时期及各个组织中呈现出多样化的表达特征,这说明不同成员可能在马铃薯生长发育过程中发挥特异的调控作用.以上结果可为进一步探索StGRF的功能提供一定基础.
Abstract
Growth-regulating factor(GRF)family genes encode plant-specific transcription factors that play critical roles in many biologi-cal processes such as plant growth and development,and stress response.In this study,bioinformatics approaches were employed for the genome-wide identification and expression pattern analysis of the GRF gene family in potato(Solanum tuberosum).A total of 12 GRF fam-ily genes(StGRF1～StGRF12)were identified in the potato genome,and their encoded proteins both contain the QLQ domains and WRC domains.The StGRF were distributed on 9 chromosomes of potato,and chromosomal segmental duplications may have contributed to the expansion of this gene family.Phylogenetic analysis showed that StGRF could be divided into 5 groups,and members from the same group exhibited similar gene structures and conserved motif distribution.Abundant elements associated with hormone and stress responsive ele-ments were found in the promoter regions of StGRF genes;Expression pattern analysis demonstrated diverse expression characteristics of StGRF across different developmental stages and tissues of potato,indicating that different members may exert specific regulatory roles during the growth and development processes of potato.The results of this study can provide a basis for further functional analysis of StGRF.
关键词
马铃薯/生长调节因子/基因家族/生物信息学/表达模式Key words
Potato/Growth-regulating factor/Gene family/Bioinformatics/Expression pattern引用本文复制引用
基金项目
国家自然科学基金(32101788)
聊城大学博士科研启动基金(318052021)
聊城大学大学生创新创业训练计划(S202110447009)
聊城大学大学生创新创业训练计划(CXCY2022338)
出版年
2024
NETL
NSTL
万方数据