Genome-wide Identification of Hsp Gene Family and Analyzing Its Expression Under Stress in Sinonvacula constricta
Heat shock proteins(Hsps),highly conserved proteins widely distributed in animals,plants and microorganisms,can be induced by biological and abiotic stress expression.In order to explore the role of Hsp in response to biotic and abiotic stresses in Si-nonovacula constricta,this study identified 62 ScHsp genes in the whole genome of S.constricta by bioinformatics methods,and predic-ted their gene structure,chromosome location and protein physical and chemical properties,and the expression patterns of ScHsp family genes in S.constricta were analyzed in different tissues and under biological(Vibrio parahemolyticus infection)and abiotic stresses(high temperature stress and dry exposure).The results showed that there were significant differences in the number of introns in differ-ent ScHsp families;the 62 ScHsp genes unevenly distributed on 17 chromosomes,and gene replication analysis revealed three sets of tandem replication genes,which may be an important reason for the number expansion of ScHsp family genes;most of the ScHsp expres-sions were tissue-specific,while a few genes(ScHsp90-2 and ScHsp70-11)showed a pan-expression pattern;ScHsp family genes showed different levels of response to Vibrio parahaemolyticus infection and high temperature stress,and divided into 6 and 4 patterns according to the trend of gene expression;the expression levels of 6 representative genes of the ScHsp gene family were analyzed by qPCR at 5 time points(0,12,24,48,72 h)under low temperature and normal temperature dry exposure.The results showed that the 6 genes responded to dry exposure to different degrees.This study provides basic information for a comprehensive and in-depth study of the mechanism of ScHsp genes in response to stress in S.constricta.
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