Effect of Growth Hormone on Chicken Fibroblast Cells Infected with Subgroup J Avian Leukosis Viruses
Growth hormone(GH)not only regulates metabolism,growth,development,and reproduction of animals,but also the im-mune system of animals.The aim of this study was to analyze key genes and signaling pathways in chicken fibroblast cells(DF-1)infec-ted with subgroup J avian leukosis viruses(ALV-J)after overexpression of GH by transcriptome sequencing and bioinformatics techno-logy.DF-1 was transfected with empty-load plasmid and GH overexpression plasmid respectively,and inoculated with ALV-J strain SCAU-HN06.Transcriptome sequencing was used to detect the changes in the gene expression profile.The results showed that com-pared with the control group,there were 122(49％)up-regulated differentially expressed genes(DEGs)and 127(51％)down-regula-ted DEGs in the experimental group.The GO enrichment analysis revealed that DEGs were primarily enriched in me-tabolic processes,biological regulation,cellular processes,cell part,catalytic activity,and binding.The KEGG enrichment analysis found that DEGs were most heavily enriched in the immune system,signal transduction,signaling mole-cular and interaction,cell growth and death pathways.Furthermore,protein-protein interaction(PPI)networks were built,and seven immune-related genes were tested using real-time quantitative PCR(RT-qPCR).The expression trends of TRAF1,DSTYK and HESX1 genes were consistent with the results of RNA sequencing(RNA-seq).In this study,the key genes and signaling pathways that may be involved in the regulation of ALV-J were dis-covered through bioinformatics analysis.It is expected to provide new insights into the relationship between virus-host interaction in avi-an leukosis,as well as new ideas for the purification of avian leukosis.
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