Mogroside Promotes Lipid Metabolism in Mus muscolus Induced by High-fat Diet
In order to study the effect of mogroside on growth performance and lipid metabolism in high-fat diet Mus muscolus,forty 21-day-old male KM Mus muscolus(weighing about 14 g)were selected in this experiment,and they were randomly divided into four treat-ment groups(n=10 animals/group),i.e.,control group(CK),low concentration sweet glycoside group(LSG),medium concentra-tion sweet glycoside group(MSG)and high concentration sweet glycoside group(HSG).The preliminary test lasted for 7 days,and the formal test lasted for 56 days.At the end of the test period,the blood glucose concentration was measured after weighing one by one,and the weights of brown adipose tissue,subcutaneous adipose tissue and liver tissues were collected and weighed;The paraffin sec-tions of brown adipose tissue and subcutaneous adipose tissue were stained with HE and the liver tissue paraffin sections were stained with oil red O.The expression of UCP1,PRDM16,PGC-1α,and the genes(AMPK,PPARγ,and C/EBPα)relating to the fat meta-bolism signaling pathway in brown adipose tissue,abdominal adipose tissue,and subcutaneous fat in the CK group and HSG group was detected by real-time quantitative PCR(RT-qPCR).The results showed that the body weight,liver weight and blood glucose of Mus muscolus fed with 100,300,600 mg·kg-1·d-1mogroside solution decreased,and the deposition of brown adipose tissue,subcuta-neous adipose tissue and liver adipose tissue in Mus muscolus fed with high-fat diet increased.RT-qPCR showed that the expression of UCP1,PRDM16,PGC-lα,AMPK,PPARγ and C/EBPα mRNA in brown adipose tissue,abdominal adipose tissue and subcutaneous adipose tissue significantly increased in HSG group(P＜0.05).This study can provide an important scientific basis for the development of mogroside and its rela-ted industries,and provide a theoretical reference for the further analysis of the molecular mechanism of mo-groside in regulating lipid metabolism.
MogrosideMus muscolusFat metabolismFat type conversion
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