首页|β-岚香酮酸对肝细胞癌细胞增殖和凋亡的影响及其机制

β-岚香酮酸对肝细胞癌细胞增殖和凋亡的影响及其机制

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本研究旨在探讨β-岚香酮酸(β-elemonic acid,β-EA)对肝细胞癌(hepatocellular carcinoma,HCC)细胞增殖和凋亡的影响及其作用机制.利用CCK8法检测β-EA对人HCC细胞Huh7和PLC/PRF/5活力的影响;利用Annexin V-FTTC/7AAD染色检测细胞凋亡变化;利用流式细胞周期检测β-岚香酮酸对HCC细胞周期的影响;利用Western blot检测凋亡相关蛋白Bcl2、BAX、cleaved-caspase 3和细胞周期相关蛋白CDK1、Cyclin B1的表达.结合网络药理学预测β-EA抑制HCC细胞增殖的分子机制,检测PI3K/AKT信号通路蛋白的表达.通过构建H22皮下移植瘤模型,体内证实了 β-EA对HCC的生长抑制作用.结果表明,β-EA能够显著抑制Huh7和PLC/PRF/5细胞活力,诱导HCC细胞凋亡和G2/M期细胞阻滞,上调促凋亡蛋白cleaved-caspase 3、BAX,下调抗凋亡蛋白Bc12、细胞周期蛋白Cyclin B1、细胞周期依赖性激酶CDK1、p-PI3K、p-AKT的表达.皮下移植瘤模型证实β-EA能够有效降低瘤重、瘤体积和Ki67的表达.本研究结果表明β-EA能够通过PI3K/AKT信号通路诱导HCC细胞凋亡和G2/M期细胞阻滞.
Effect of β-elemonic Acid on Proliferation and Apoptosis of Hepatocellular Car-cinoma Cells and Its Mechanism
The aim of this study was to investigate the effect of β-elemonic acid(β-EA)on hepatocellular carcinoma(HCC)prolifera-tion and apoptosis and its mechanism.CCK8 assay was used to detect the effects of β-EA on the cell viability of human HCC cells Huh7 and PLC/PRF/5.Annexin V-FTTC/7AAD staining was used to detect cell apoptosis.The effect of β-EA on cell cycle of HCC cells was detected by flow cytometry.The expressions of apoptosis-related proteins Bc12,BAX,cleaved-caspase 3 and cell cycle related pro-teins CDK1 and Cyclin B1 were detected by Western blot.The molecular mechanism of β-EA inhibiting the proliferation of HCC cells was predicted by network pharmacology,and the protein expression of PI3K/AKT signaling pathway was detected.The growth inhibi-tion effect of β-EA on HCC was confirmed by constructing H22 subcutaneous transplantation tumor model in vivo.The results showed that β-EA significantly inhibited Huh7 and PLC/PRF/5 cell viability,induced cell apoptosis and G2/M cell arrest.The expression of pro-apoptotic protein cleaved-caspase 3 and BAX was up-regulated,and the expression of anti-apoptotic protein Bc12,cell cycle protein Cyclin B1,cycle-dependent kinases CDK1,p-PI3K,and p-AKT was down-regulated.The subcutaneous tumor transplantation model confirmed that β-EA can effectively reduce tumor weight,tumor volume,and Ki67 expression.The results of this study suggest that β-EA can induce apoptosis and G2/M cell arrest of HCC cells through PI3K/AKT signaling pathway.

Hepatocellular carcinomaβ-elemonic acidCell proliferationCell apoptosisCell cycle arrest

邹晨、乔凤杰、方淼、高亚婷、马言璐、高月求、张鑫

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上海中医药大学附属曙光医院细胞免疫实验室,上海市中医临床重点实验室,上海,201203

上海中医药大学附属曙光医院肝病科,上海,201203

肝细胞癌 β-岚香酮酸 细胞增殖 细胞凋亡 细胞周期阻滞

国家自然科学基金上海中医药大学"杏林百人"人才项目上海市临床重点专科建设项目(中医专业)上海市名老中医学术经验研究工作室项目中医药领军人才项目岐黄学者支持项目

81973773shslczdzk01201SHGZS-202246

2024

10.13417/j.gab.043.000907

基因组学与应用生物学
广西大学

基因组学与应用生物学

CSTPCD北大核心
影响因子：1.108
ISSN：1674-568X
年,卷(期)：2024.43(5)
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