首页|不同生长阶段伊拉兔骨骼肌转录组数据的挖掘分析

不同生长阶段伊拉兔骨骼肌转录组数据的挖掘分析

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为了进一步挖掘伊拉兔骨骼肌转录组的数据,本研究运用生物信息学对3个生长阶段的伊拉兔腓肠肌转录组数据进行深入的综合分析.通过对可变剪切、新转录本及其转录因子的表达模式和分子功能进行可视化呈现,结果发现,在伊拉兔骨骼肌的生长过程中,外显子跳跃(skipping exon,SE)是最常见的剪切方式,发生可变剪切的基因主要富集于泛素化介导的蛋白水解过程.同时,挖掘出4个新转录本,其编号分别是Novel.10047、Novel.13070、Novel.16785和Novel.7389,均与骨骼肌的氧化-还原过程和线粒体功能相关.同属于zf-C2H2转录因子家族成员的转录因子PLAGL1、KLF15、IKZF2和ZNF566可能作为潜在靶点参与调控伊拉兔骨骼肌的生长过程.研究结果对伊拉兔基因组和转录组注释信息进行了补充,为进一步完善及解析伊拉兔骨骼肌生长过程中潜在的功能基因及作用提供了理论基础和依据.
Mining Analysis of Skeletal Muscle Transcriptome Data of Ira Rabbits at Differ-ent Growth Stages
To further mine the transcriptome data of the skeletal muscle of Ira rabbits,bioinformatics was used to conduct an in-depth comprehensive analysis of the transcription data of the gastrocnemius muscle of Ira rabbits at three growth stages in the study.The ex-pression patterns and molecular function of alternative splicing,new transcripts,and their transcription factors were visualized.The re-sults showed that skipping exon(SE)was the most common splicing mode in skeletal muscle growth of Ira rabbits,and the genes for al-ternative splicing were mainly enriched in ubiquitination-mediated proteolysis.Meanwhile,four new transcripts codes were Novel.10047,Novel.13070,Novel.16785,and Novel.7389 respectively,which were mainly related with the oxidation-reduction process and mitochondrial function in skeletal muscle.Transcription factors including PLAGL1,KLF15,IKZF2,and ZNF566 belonged to the zf-C2H2 transcription factor family may be involved in the regulation of skeletal muscle growth in Ira rabbits as potential targets.The re-sults supplemente the genome and transcriptome annotation information of Ira rabbits and provide a theoretical foundation and basis for further improving and analyzing the potential functional genes and their roles in the skeletal muscle growth of Ira rabbits.

Alternative splicingTranscriptsTranscription factorsTranscriptomeIra rabbit

吴倩雯、王露、王文佳、姬正伟、路宏朝、程佳

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陕西理工大学生物科学与工程学院,汉中,723001

秦巴特色肉制品质量提升与安全控制陕西省高校工程研究中心,汉中,723001

秦巴生物资源与生态环境省部共建国家重点实验室(培育),汉中,723001

可变剪切 转录本 转录因子 转录组 伊拉兔

秦巴生物资源与生态环境省部共建国家重点实验室(培育)"市校共建"科研专项国家自然科学基金项目

SXC-210132272884

2024

基因组学与应用生物学
广西大学

基因组学与应用生物学

CSTPCD北大核心
影响因子:1.108
ISSN:1674-568X
年,卷(期):2024.43(6)
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