Regulatory Mechanism of Sophora subprosrate Polysaccharide on Inflammatory Response of Murine Spleen Lymphocytes
The study aimed to investigate the regulatory mechanism of Sophora subprosrate polysaccharide(SSP)on the inflammatory response of murine spleen lymphocytes based on transcriptome sequencing technology.Primary murine spleen lymphocytes were used to set up the cell control group and the 400 μg/mL SSP treatment group.Based on the whole transcriptome sequencing data of Illumina NovaSeq 6000 sequencing technology,the differentially expressed mRNA,lncRNA,and miRNA were analyzed by DeSeq 2.0 software.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)enrichment were used to analyze the function of differen-tially expressed genes(DEGs).The mRNA,lncRNA,and miRNA related to the inflammatory response were randomly selected from the DEGs,and the sequencing results were verified by RT-qPCR.The results of transcriptome sequencing showed that a total of 658 DEGs were obtained in the 400 μg/mL SSP treatment group,of which 373 were up-regulated and 285 were down-regulated when com-pared with the cell control group.GO function and KEGG pathway enrichment analysis showed that the DEGs were related to immune and inflammatory responses.The DEGs were mainly enriched in the nucleotide-binding oligomerization domain(NOD)-like receptor signaling pathway,nuclear factor kappa-B(NF-κB)signaling pathway,retinoic acid-inducible gene-I(RIG-I)-like signaling pathway,regulation of stem cell pluripotency signaling pathway,forkhead box O(FOXO)signaling pathway,chemokine signaling pathway,mi-togen activated protein kinase(MAPK)signaling pathway,and C-type lectin receptor signaling pathway.The results of RT-qPCR were consistent with the gene expression levels of whole transcriptome sequencing.Through the analysis of transcriptome DEGs,it was found that SSP may regulate the inflammatory response of the body through downstream inflammatory signaling pathways and immune-related pathways such as the NF-κB signaling pathway and NOD-like receptor signaling pathway.This study provides a certain reference for the development and application of SSP.
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