Analysis of Genetic Diversity of Neuroctenus taiwanicus Based on COI Gene Se-quence
The COI gene sequence of mtDNA was used as a molecular marker to analyze the genetic diversity,genetic differentiation,and geographic pattern of 16 populations of Neuroctenus taiwanicus.According to the sequencing results,the COI gene of N.taiwanicus was 1 536 bp in length,which showed a significant A/T base bias.There were 27 haplotypes,2 shared haplotypes and 25 exclusive haplotypes in 16 geographic populations,and the population did not expand.Most geographic populations had little gene exchange.The population can be divided into two main groups,one in Yunnan and Guangxi,and the other in Hainan,Guizhou,Fujian,and Laos Houaphan.However,some of the samples from Hainan,Laos Houaphan and Yunnan were clustered together,which may be related to the formation of Hainan island drift.Based on the analysis of a phylogenetic tree and median-joining network,the results suggested that Menglun Town in Yunnan Province may be one of the birthplaces or the closest to the birthplaces.