基于COI基因序列的台湾脊扁蝽遗传多样性分析
Analysis of Genetic Diversity of Neuroctenus taiwanicus Based on COI Gene Se-quence
贾战成 1张学成 1苏日娜 2白晓拴1
作者信息
- 1. 内蒙古师范大学生命科学与技术学院,呼和浩特,010022
- 2. 西北农林科技大学植物保护学院,咸阳,712100
- 折叠
摘要
本研究以mtDNA中的COI基因序列作为分子标记,对 16 个种群的台湾脊扁蝽(Neuroctenus taiwanicus)进行了遗传多样性、遗传分化和地理格局等分析.测序结果显示,台湾脊扁蝽COI基因序列长度为1 536 bp,具有明显的A/T碱基偏倚性.16 个种群的台湾脊扁蝽产生了 27 个单倍型,其中,共享单倍型 2 个,独享单倍型 25 个,种群未发生扩张;变异来源主要是种群间,且遗传分化程度高,大部分种群基因交流较少;种群可分为两大支系,云南省和广西壮族自治区的种群为一支,海南省、贵州省、福建省和老挝华潘省的种群为另一支.但其中海南省、老挝华潘省种群中部分样本与云南省的种群样本聚在一起,此结果推测与海南岛的漂移形成有关.根据系统发育树和单倍型中介网络图综合分析结果,推测云南省的勐仑镇可能是台湾脊扁蝽的发源地之一或最接近发源地.
Abstract
The COI gene sequence of mtDNA was used as a molecular marker to analyze the genetic diversity,genetic differentiation,and geographic pattern of 16 populations of Neuroctenus taiwanicus.According to the sequencing results,the COI gene of N.taiwanicus was 1 536 bp in length,which showed a significant A/T base bias.There were 27 haplotypes,2 shared haplotypes and 25 exclusive haplotypes in 16 geographic populations,and the population did not expand.Most geographic populations had little gene exchange.The population can be divided into two main groups,one in Yunnan and Guangxi,and the other in Hainan,Guizhou,Fujian,and Laos Houaphan.However,some of the samples from Hainan,Laos Houaphan and Yunnan were clustered together,which may be related to the formation of Hainan island drift.Based on the analysis of a phylogenetic tree and median-joining network,the results suggested that Menglun Town in Yunnan Province may be one of the birthplaces or the closest to the birthplaces.
关键词
台湾脊扁蝽/COI序列/扁蝽/遗传分化/遗传多样性Key words
Neuroctenus taiwanicus/COI sequence/Aradidae/Genetic differentiation/Genetic diversity引用本文复制引用
基金项目
国家自然科学基金项目(32060121)
内蒙古师范大学研究生科研创新基金项目(CXJJS22122)
出版年
2024
NETL
NSTL
万方数据