表达IBDV VP2基因的重组嵌合型新城疫病毒的免疫原性初步评价
Immunogenicity Evaluation of Recombinant Chimeric Newcastle Disease Virus Expressing IBDV VP2 Gene
周俏艳 1杨宏春 1陈浩 1冯书风 1李海珠 1刘耕松 1欧长波2
作者信息
- 1. 广西大学动物科学技术学院,南宁,530004
- 2. 广西大学动物科学技术学院,南宁,530004;广西壮族自治区兽用生物制品工程研究中心,南宁,530004;广西畜禽繁育与疾病防控重点实验室,南宁,530004
- 折叠
摘要
本研究为了研发能同时预防传染性法氏囊病病毒(infectious bursal disease virus,IBDV)和新城疫病毒(Newcastle dis-ease virus,NDV)感染的二联苗,将IBDV VP2 基因插入NDV LaSota疫苗株基因组的P基因和M基因之间,利用NDV反向遗传操作系统构建并成功获得重组病毒LS-IBDV-VP2.对重组病毒的鸡胚平均死亡时间(mean death time,MDT)、鸡胚半数感染量(egg infectious dose,EID50)和 1 日龄雏鸡脑内接种的致病指数(intracerebral pathogenicity index,ICPI)等生物学特性进行测定,并对 18 日龄SPF鸡胚进行免疫接种,利用血凝抑制实验(hemagglutination inhibition test,HI)测定NDV抗体以及间接酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)测定IBDV VP2 抗体,评价重组病毒的免疫应答反应.结果显示,重组病毒LS-IBDV-VP2 的EID50 为108.5/mL、MDT为147 h、ICPI为0;NDV抗体效价在孵化后第14 天达到最高,与La-Sota疫苗株差异不显著(P>0.05),并在孵化后第 14 天也能产生较高水平的IBDV VP2 抗体.本研究结果表明,重组病毒LS-IBDV-VP2 与LaSota疫苗株具有相似的生物学特性,但重组病毒毒力更低,并且能够有效诱导机体产生抗体,具有成为预防IBDV和NDV二联候选疫苗株的潜力.
Abstract
In order to develop a vaccine that can simultaneously prevent infection by infectious bursal disease virus(IBDV)and New-castle disease virus(NDV),the IBDV VP2 gene was inserted between the P gene and the M gene of the NDV LaSota vaccine strain ge-nome,and the recombinant virus LS-IBDV-VP2 was constructed and obtained successfully by using the NDV reverse genetic operating system.Mean death time(MDT),egg infectious dose(EID50)and intracerebral pathogenicity index(ICPI)of 1-day-old chicks inocu-lated with recombinant virus and other biological characteristics were determined,and the 18-day-old SPF chicken embryo was immu-nized,NDV antibody was determined by hemagglutination inhibition test(HI)and IBDV VP2 antibody was determined by indirect en-zyme linked immunosorbent assay(ELISA),and the immune response of recombinant virus was evaluated.The results showed that the EID50 of LS-IBDV-VP2 was 108.5/mL,the MDT was 147 h and the ICPI was 0.The titer of NDV antibody reached the highest at 14 days after hatching,which was not significantly different from that of LaSota vaccine strain(P>0.05),and a higher level of IBDV VP2 antibody was also produced at 14 days after hatching.The results of this study indicate that the recombinant LS-IBDV-VP2 has similar biological characteristics to the LaSota vaccine strain,but its recombinant virus has lower virulence and can effectively induce the body to produce antibodies,which has the potential to be a candidate vaccine strain for the prevention of IBDV and NDV.
关键词
传染性法氏囊病病毒(IBDV)/新城疫病毒(NDV)/VP2基因/反向遗传操作系统/鸡胚免疫Key words
Infectious bursal disease virus(IBDV)/Newcastle disease virus(NDV)/VP2 gene/Reverse genetic operating system/Chick embryo immunity引用本文复制引用
基金项目
广西科技基地和人才专项项目(桂科AD22035170)
出版年
2024
NETL
NSTL
万方数据