Analysis of Genomic and Basic Biological Characterization of Bacillus anthracis
In order to conduct in-depth research on the genome information of Bacillus anthracis and explore its secondary metabolites,drug-resistance genes and virulence gene types,the genomes of 43 strains of B.anthracis were downloaded from the NCBI database and genomic analysis software,secondary metabolite mining software,and drug-resistance and virulence gene databases were used to ana-lyze the genome information.The genome size of 43 strains of B.anthracis ranged from 5.20 to 5.32 MB,and the G+C content ranged from 35.36%to 35.45%.All strains could be divided into three branches on the phylogenetic tree.The pangenomic analysis showed that there were 7 873 gene families,3 469 core genes and 2 627 specific genes,and the pangenome was open.In total 12 categories,307 gene clusters related to secondary metabolites and 354 secondary metabolites were annotated through secondary metabolite mining software,mainly including linear azol in e-containing peptides(LAP),NI-siderophore,non ribosome peptide metallophore(NRP-metal-lophore),non ribosome peptide synthetase(NRPS),betalactone and terpene.By uploading genomic sequences of 43 strains of B.an-thracis in fasta format to the CARD database for sequence comparison,8 drug-resistance genes were identified,which were VanZF,mphL,FosB,Bla1,Bla2,tet45,VanRA and VanSA.Strain HDZK-BYSB7 and strain MCCC 1A01412 had the most drug-resistance genes.By uploading genomic sequences of 43 strains of B.anthracis in fasta format to the VFDB database for sequence comparison,9 virulence genes were obtained,which were inhA,nheA,nheB,nheC,BAS3109,cytk,hblA,hblC,and hblD.Strain MCCC 1A02161 had the most virulence genes,and inhA and BAS3109 might be unique virulence genes of B.anthracis compared to other Bacillus spe-cies.This study obtains the genomic information of B.anthracis,clarifies the relationship between pangenome,core genome and ge-nome number,predicts secondary metabolites and the types of drug-resistance genes and virulence genes.The research results help us comprehensively understand B.anthracis,and provides a theoretical basis for clinical medication.
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