Exploring the Potential Mechanisms of Yiyi Fuzi Baijiangsan on Regulating the Ferroptosis of Ulcerative Colitis via Network Pharmacology and Bioinformatics
The purpose of this study was to explore the potential molecular mechanism of YYFZBJS(Yiyi Fuzi Baijiangsan)in regula-ting ferroptosis in the treatment of ulcerative colitis(UC)by bioinformatics method.The components and action targets of YYFZBJS were obtained by the method of network pharmacology,and then intersected with UC disease genes and ferroptosis related genes.The differential gene expression of"YYFZBJS-UC-ferroptosis"was extracted by GSE206285 data set,and the correlation analysis,KEGG and GO analysis were carried out.LASSO and SVM machine learning methods were used to further screen the key differential genes and analyze them by gene set enrichment analysis(GSEA)and gene set variation analysis(GSVA)to evaluate the differences in the ac-tion pathways of these genes in different expression.Secondly,the key genes were analyzed by immune infiltration analysis and correla-tion analysis,the ceRNA regulation map was established,the relationship between lncRNA-miRNA-mRNA was explored,and the ac-curacy of the above genes was verified by GSE87473 verification set,and degree sequencing was carried out by Cytoscape 3.9.1 soft-ware.Finally,molecular docking was used to further verify the reliability of related targets.A total of 27"YYFZBJS-UC-ferroptosis"differential genes were obtained.It was found that the key genes were closely related to M0 macrophages,M2 macrophages,neutrophils and so on.The verification set found that there were still significant differences in BRD2,HIF-1A,IDH1,PPARG and PPARA.The first three genes HIF-1A,PPARG and NQO1 were obtained by degree sequencing.The results of molecular docking showed that stig-masterol,benzoylaconitine and isoorientin had stable binding effect with HIF-1A and PPARG.To sum up,this study found that the mechanism of YYFZBJS regulating ferroptosis treatment of UC may be through the regulation of HIF-1A,PPARG and other genes to play a role in drug effect,which provides a new reference for the treatment of UC.
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