Yiqi Huoxue Powder Intervention in Sepsis:a Bioinformatics Study of Key Genes and Core Pathways
This work used bioinformatics methods to screen for critical genes,and predict the primary pathways involved in sepsis.First,the Materia Medica HERB platform was explored to find the ingredients and targets of Yiqi Huoxue powder.Afterward,several illness registries were used to screen for conceivable relevant targets of sepsis.The STRING database was used to construct the protein-protein interaction(PPI)network;PANTHER was used to annotate proteins;ChEA3 platform was used to predict transcription factors and analyze co-expression modules;Network Analyst database was utilized to create the interaction network between transcription fac-tors and miRNA.The MSigDB database was used to visualize the functional enrichment and pathway annotation analysis of genes.Using CytoHubba as a platform to construct the hub gene expression matrix.The receiver operating characteristic(ROC)curve was pro-duced for analysis,and the hub gene regulation grid was identified using this platform.The public sepsis samples GSE28750 and GSE54514 were obtained via the GEO database.Eight hub genes and a total of twenty-one active components were evaluated in this in-vestigation for the intervention of sepsis.Yiqi Huoxue powder may have a beneficial impact on sepsis by means of pathways such as the HIF-1 signaling pathway,MARK signaling pathway,necroptosis,etc.Effective biological processes that may be involved in the re-sponse to oxygen containing compound,regulation of cell death,and response to cytokine.By employing bioinformatics and machine learning models validation methods of analyzing the expression of core genes,this study advanced the comprehension of Yiqi Huoxue powder's mechanism of action in managing sepsis.Furthermore,the study identified potential gene targets and proposed ideas for trea-ting sepsis with traditional Chinese medicine.
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