Transcriptome Comparative Analysis of Two Brassica napus Varieties With Differences in Dense Plant Tolerance Under Different Planting Densities
For rapeseed(Brassica napus),the ideal plant density is important for yield improvement.To explore density responses of rapeseed,this study focused on a transcriptome analysis of upper and lower leaves of density-tolerance rapeseed varieties and non densi-ty-tolerance rapeseed varieties under normal and high plant densities.After comparative analysis,94 differentially expressed genes in the upper leaves and 407 differentially expressed genes in the lower leaves of both varieties were selected.The expression of these genes in both varieties showed differences under different planting densities.Through weighted gene coexpression network analysis(WGCNA)analysis,blue and purple modules significantly positively correlated with density tolerant rapeseed were screened.GO enrichment ana-lysis was performed on the genes in the modules,and 12 core transcription factors(including EIN3/EIL1,HD-ZIP,bHLH,NAC,ERF,and TCP)and 1 blue light receptor gene CRY2 were selected from hub genes.Functional annotation showed that multiple core genes corresponding to Arabidopsis thaliana homo-logous genes participated in shade avoidance response.This study can provide data reference and candidate gene resources for improving the density tolerance of rapeseed.
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