萜烯类化合物是茉莉香气成分的重要组成部分。萜烯类合成酶(terpene synthase，TPS)在萜烯类化合物的生物合成中发挥重要作用。为揭示TPS在茉莉香气合成中的调控作用，本研究对虎头茉莉(Jasminum sambac，'Grand Duke of Tuscany')的TPS基因家族进行了全基因组鉴定及特征分析。在虎头茉莉基因组中共鉴定到了 53个TPS基因，可将其分为5个亚家族TPS-a、TPS-b、TPS-c、TPSe'和TPS-g。系统发育、基因结构和保守基序分析表明，虎头茉莉TPS基因具有相对保守的基因结构。在TPS基因的启动子区存在多种不同功能的顺式作用元件，表明该家族成员的功能多样性。共线性结果显示，虎头茉莉中共有18对复制事件，净化选择是驱动虎头茉莉TPS基因进化的主要动力。种间共线性分析揭示了 TPS基因在不同茉莉基因型中的演化模式和历史。转录组和RT-qPCR结果显示，TPS基因家族成员在虎头茉莉不同组织中表现出特定的表达模式，并在开花不同时期中呈现先上升后下降的表达趋势。特别地，有6个TPS基因在初开期(F2)或盛开期(F3)显示出较高的表达量，这与虎头茉莉萜烯类花香物质的释放规律相一致。本研究为后续不同香型茉莉香气合成途径的研究及其资源的有效利用奠定了基础。
Identification and Expression Analysis of the Terpene Synthase Gene Family in"Hutou"Jasmine(Jasminum sambac,MP-"Hutou")During the Flowering Process
Terpenes are crucial components of the jasmine fragrance profile.Terpene synthases(TPS)play a vital role in the biosyn-thesis of terpenoid compounds.To elucidate the regulatory role of TPS in jasmine fragrance synthesis,this study carried out a compre-hensive genome-wide identification and characterization of the TPS gene family in"Hutou"jasmine(Jasminum sambac,'Grand Duke of Tuscany').A total of 53 TPS genes were identified within the genome of"Hutou"jasmine,which were further classified into five subfamilies:TPS-a,TPS-b,TPS-c,TPS-e/f,and TPS-g.Phylogeny,gene structure,and conserved motif analyses indicated that the TPS genes of"Hutou"jasmine had a relatively conserved gene structure.The presence of diverse cis-acting elements with different functions in the promoter regions of TPS genes suggested functional diversity within this gene family.Collinearity results revealed 18 duplication events within"Hutou"jasmine genome,with negative selection being the primary driving force behind the evolution of the TPS genes in this species.Interspecific collinearity analysis shed light on the evolutionary patterns and history of TPS genes across dif-ferent jasmine genotypes.Transcriptomic and RT-qPCR results demonstrated tissue-specific expression patterns of TPS gene family members in"Hutou"jasmine and displayed an expression trend of initial increase followed by a decrease during different flowering sta-ges.Notably,six TPS genes showed higher expression levels during the initial opening period(F2)or blooming period(F3),which is consistent with the release pattern of terpene floral scent compounds in"Hutou"jasmine flower.This study lays the foundation for fur-ther research into the synthesis pathways of different jasmine fragrance types and the effective utilization of their resources.
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