Identification and Expression Analysis of Stress Associated Protein Gene Family in Panax ginseng
Panax ginseng is a perennial herb of the Panax genus in the family Acoraceae,which is a traditional precious Chinese me-dicinal material.Ginseng is often affected by the late spring coldness in the growth and development process,and the cold damage of ginseng seriously affects the yield and quality of ginseng.Stress associated protein(SAP)is mainly involved in a variety of abiotic stresses in plants,such as cold,drought and salt stress.This study was based on the ginseng transcriptome database and identified a total of 60 SAP gene family genes in ginseng.It used bioinformatics methods to predict the physical and chemical properties,and chro-mosomal location information.The conserved motifs,conserved domains,colinearity,systematic evolution,cis-regulatory elements,GO function annotation,and expression patterns of the gene family were analyzed.The results indicate that members of the PgSAP gene family are structurally stable,evolutionarily conserved,and spatiotemporally and temporally expression-specific,with cis-acting ele-ments related to hormones and associated with defence and stress predicted in their upstream 2 000 bp regulatory sequence.Then,five genes related to saponin synthesis were screened out according to SNP mutations,and the expression levels of these genes were verified under 4℃ cold stress.The results showed that all five PgSAP genes responded to low-temperature stress and were closely related to gin-senoside synthesis,providing a theoretical basis for further in-depth study of important gene families in ginseng.
Panax ginsengSAP gene familyLow temperature stressBioinformaticsExpression analysis
NETL
NSTL
万方数据
