Comparative Transcriptome Analysis of Muscle,Heart,and Liver of Banjiao Goat and Youzhou Dark Goat
Banjiao goat and Youzhou dark goat are local characteristic breeds of goats(Capra hircus)in Chongqing.The purpose of this study was to study the gene differential expression of Banjiao goat and Youzhou dark goat.In this study,Illumina sequencing tech-nology was used to perform transcriptome sequencing on longissimus dorsi muscle,heart,and liver from Banjiao goat and Youzhou dark goat.Sequence alignment,structural analysis,and transcription factor prediction were performed,and differentially expressed genes(DEGs)were screened for functional enrichment analysis.Six DEGs were randomly selected for RT-qPCR verification.Analysis of the results showed that 1 390 854 single nucleotide polymorphism(SNP)sites,74 758 insertion/deletion(Indel),and 53 625 alternative splicing were detected in the heart,longissimus dorsi muscle,and liver tissues from Banjiao goat and Youzhou dark goat.66,190,and 377 DEGs were identified in differential expression analysis,and these genes were divided into 14 transcription factor families.The DEGs were also involved in several metabolic pathways related to muscle composition,organic acid and fatty acid metabolism.This study found that the ASIP gene in goats may affect meat quality by partici-pating in the regulation of fat metabolism in different tissues.The ANKRD1 gene could be another important candidate gene that inf-luencing the differences in meat quality between Banjiao goat and Youzhou dark goat.The DNAJB1,MTL5,and N2DL2 gene may be involved in the immune regulation of different breeds of goats.This study elucidates the gene expression difference between Banjiao goat and Youzhou dark goat,which provides important information for the mining and utilization of genetic resources of Banjiao goat and Youzhou dark goat.
Banjiao goatYouzhou dark goatTranscriptome analysisGene expression
NETL
NSTL
万方数据
