Analysis of Transcription Characteristics of Muscle and C2C12 Cells in Mstn Gene Knock-out Mice
Myostatin(Mstn),a negative regulator of skeletal muscle growth and development within the TGF-β superfamily member,plays a crucial role in modulating the development of skeletal muscles.Myostatin can impact muscle development,manifesting pheno-typically as traits such as double-muscling in individuals.To investigate the impact of Mstn gene knock-out on the muscle transcriptome levels,this study applied CRISPR/Cas9 technology in generating three strains of C2C12 cell with Mstn gene knock-out.Additionally,the gastrocnemius muscles from 12 Mstn gene knock-out mice(6 males,6 females)were collected for transcriptome sequencing.The results showed that the proliferative capacity of Mstn gene knock-out cells was significantly higher than that of wild-type cells.Further-more,transcriptome sequencing revealed that 329,240,265 differentially expressed genes(DEGs)were found in the cells,male mice,and female mice,respectively.50 DEGs were both identified in Mstn gene knock-out male and female mice.Among the down regulated genes,Myh7,Lmod2,Barx2,Myh2,Myl3,and Smtnl1 genes were significantly enriched in 27 muscle function related pathways.Dgat2 and Slc27a1 were significantly enriched in 8 lipid metabolism related pathways.In addition,4 DEGs were identified in Mstn gene knock-out cells and mice,among which the Fam83d gene was a key regulatory gene for myoblast proliferation and differentia-tion.Above all,Mstn gene knock-out can participate in muscle development regulation by down regulated genes such as Myh7,Lmod2,and Fam83d,as well as regulating lipid metabolism by down regulated genes Dgat2 and Slc27a1.
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