Mitochondrial Genome Characteristics and Phylogenetic Analysis of Triplophysa weiheensis
Triplophysa weiheensis is a special fish distributed in the upstream of the Yellow River.The current study attempted to de-pict the structural characteristics of the mitochondrial genome of Triplophysa weiheensis and to explain the phylogenetic relationship of this species in Triplophysa.The high-throughput sequencing technology was applied to obtain the complete mitochondrial genome se-quence of T.weiheensis.Furthermore,a phylogenetic tree of T.weiheensis and 13 Triplophysa fishes was constructed based on the mi-tochondrial genome using the maximum likelihood method(ML).The results showed that the total length of the T.weihensis mitochon-drial genome was 16 570 bp,including 13 protein-encoding genes,22 tRNA genes,2 rRNA gene,and 2 non-encoding areas(D-loop and OL).The D-loop region had the highest A+T content(65.87％).ATG were the most common start codons in the protein-coding gene,and the coxl gene was the only exclusion starting with GTG.Among the 22 tRNA genes,21 tRNA genes exhibited a typical clo-ver structure,while tRNASer(GCU)was in the absence of a DHU arm,leading to the lack of the typical clover shape.The phylogenetic tree constructed based on protein-coding genes using the maximum likelihood method(ML)demonstrated that T.weiheensis was closely related to Triplophysa stoliczkai and Triplophysa sellaefer.This study supplements the basic data of mitochondrial genome of T.weiheen-sis,which is helpful to deepen the understanding of the phylogenetic relationship of Triplophysa.The evolutionary relationship between different species of Triplophysa was clarified,which laid a solid foundation for the subsequent research on adaptive evolution and selec-tion pressure of Plateau fish.
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