Genetic Diversity and Phylogenetic Relationship of Rhinogobio nasutus in the Up-per Reaches of the Yellow River Based on Mitochondrial Cytb and CO Ⅰ Genes
In order to study the genetic structure and phylogenetic relationship of Rhinogobio nasutus in different populations in the up-per reaches of the Yellow River and protect its wild germplasm resources,the genetic diversity and phylogenetic relationship of four populations in the Ningxia section and Inner Mongolia section of the Yellow River were analyzed based on mitochondrial Cytb and CO Ⅰgenes.The results showed that the sequence length of Cytb and CO Ⅰgenes was 1 145 bp and 749 bp,respectively,and the combined sequence length of the two sequences was 1 894 bp,showing an obvious AT bias in base composition.The mitochondrial Cytb and CO Ⅰ sequences in the four populations had 8 combined polymorphic sites and 9 combined haplotypes,including a mean haplotype di-versity(Hd)of 0.776 and mean nucleotide diversity(π)of 0.000 78.Analysis of molecular variance(AMOVA)showed that there was no significant genetic differentiation among the four populations,and 95.81％of the variation came from within the populations.4.18％of the variation was among the populations.The phylogenetic tree and haplotype network map indicated that the genetic struc-ture of Rhinogobio nasutus was relatively simple,and the results of the mismatch distribution indicated that there had been a population expansion event in the history of Rhinogobio nasutus.In summary,the genetic structure of the population of Rhinogobio nasutus in the middle and upper reaches of the Yellow River is single,and the genetic diversity is low.It is necessary to conserve the population as a whole in situ,so as to enrich the genetic diversity of the population.
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