Growth Inhibition Effect of Extracts from Walnut Green Husk on Tobacco Virus
To explore the different solvent extracts from walnut green husk on tobacco virus growth inhibitory effect. [Methods] Taking Shangluo local production of walnut green husk as raw material, using ethyl ether, chloroform, ethanol, ethyl acetate and acetone five solvent extracts were prepared by refluxing method. The extract was prepared by reflux extraction method, and then the growth inhibition effect of different solvent extracts on tobacco mosaic virus and cucumber mosaic virus was determined by half leaf method. [Results] In the test conditions of different solvent extracts from walnut green husk on tobacco mosaic virus and tobacco cucumber mosaic virus has certain inhibitory effect, and with the increasing concentration of the extracts showed a good dose-response relationship. The ethyl acetate extract effect best, the highest inhibition rate could reach 65.9%, 52.3%, almost no the obvious difference with the positive control of the same concentration. [Conclusion] The ethyl acetate extract of walnut green husk has good control effect on tobacco virus.
walnut green huskextracttobacco mosaic viruscucumber mosaic virusgrowth inhibition