The effect of IL-22 on the expression of NLRP3 and caspase-1 mRNA and secretion of IL-18 and IL-1β in macrophages induced by lipopolysaccharide
Objective To explore the effect of interleukin(IL)-22 on the expression of nucleotide binding oligomerization domain like receptor protein 3(NLRP3)and caspase-1 mRNA and secretion of IL-18 and IL-1β in macrophages induced by lipopolysaccharide(LPS),RAW264.7 macrophages were cultured in vitro.Methods Macrophage RAW264.7 was cultured in vitro,and the cultured cells were divided into three groups(control group,LPS group and LPS+IL-22 group),and the experimental cells in each group were intervened,and cultured for 3,6 and 24 h respectively,and the cells and supernatants in each group were collected.RT-PCR,Western Blot and ELISA were used to detect NLRP3 and caspase-1 when the inflammatory body of macrophage NLRP3 was activated.Results The expression levels of NLRP3 and caspase-1 mRNA and the secretion levels of IL-1β and IL-18 were increased in the LPS group,and the differences were statistically significant compared with the control group.After LPS and IL-22 co-stimulated macrophages,the expression levels of NLRP3 and caspase-1 mRNA,and the secretion levels of IL-1β and IL-18 were increased to different degrees,which were significantly increased compared with the LPS group.Conclusion IL-22 could provide a new therapeutic idea for sepsis by enhancing the expression of NLRP3 and caspase-1 mRNA and the secretion of IL-18 and IL-1β in macrophages induced by LPS.
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维普
