Objective To explore the mechanism by which Pien Tze Huang improves liver Kupffer cell damage induced by lipopolysaccharide(LPS)by regulating the expression of miR-155.Methods LPS induced liver Kupffer cells to establish a cell injury model to simulate septic liver injury.RT-qPCR was used to detect the expression of miR-155 in damaged cells,and RT-qPCR,Western Blot,ELISA and flow cytometry were used to evaluate the inflammatory response and apoptosis of damaged cells.Then we treated LPS-induced Kupffer cells with Pien Tze Huang at different concentrations(0 mg/L,5 mg/L,10 mg/L and 15 mg/L),and detected the expression of miR-155 in the cells,the inflammatory response of the cells and Apoptosis rate.MiR-155 was silenced in the cell injury model,and RT-qPCR,Western Blot,ELISA and flow cytometry were used to evaluate the effect of miR-155 on inflammatory response and apoptosis of model cells.Overexpression of miR-155 in damaged cells treated with Pien Tze Huang was used to detect changes in cellular inflammatory response and apoptosis.Data are expressed in the form of mean±standard deviation,and each group of data is analyzed using t test or one-way analysis of variance.Results In the LPS-induced liver Kupffer cell injury model,the expression of miR-155 was significantly increased(P＜0.05),the expression levels of pro-inflammatory factors IL-6 and TNF-α were significantly increased,and the anti-inflammatory factor IL-10 was significantly increased.was inhibited(P＜0.05),and the cell apoptosis rate was significantly increased(P＜0.05).After Pien Tze Huang treatment,the expression of miR-155 in damaged liver cells was inhibited(P＜0.05),the levels of cellular inflammatory factors IL-6 and TNF-α were inhibited,and the expression of anti-inflammatory factor IL-10 was promoted(P＜0.05).Inhibit cell apoptosis(P＜0.05).Silencing miR-155 reduced the inflammatory response and apoptosis rate of cells(P＜0.05).Overexpression of miR-155 can reverse the effect of Pien Tze Huang on liver cell injury(P＜0.05).Conclusions In the model of LPS-induced liver Kupffer cell injury,Pien Tze Huang can inhibite the inflammatory response and apoptosis of cells by inhibiting the expression of miR-155.
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