Expression of miR-34a and TNF-α in neonatal rat model of acute respiratory distress syndrome lung injury
Objective To investigate the expression of microRNA-34a(miR-34a)and tumor necrosis factor-α(TNF-α)in lung injury induced by lipopolysaccharides(LPS)in neonatal rats with acute respiratory distress syndrome(ARDS).The expression of mirna-34a and tumor necrosis factor-α(TNF-α)in lung injury induced by lipopolysaccharides(LPS)was determined by tumor necrosis factor.Methods A total of 807-day-old newborn SD rats were randomly(random number)divided into two groups,40 in each group,NARDS animal models were established by intraperitoneal injection of 4 mg/kg LPS solution for 3,6,12 and 24 hours respectively.Normal control group was established by intraperitoneal injection of 4 mL/kg isotonic Nacl solution.The wet/dry weight ratio of lung was measured to observe the changes of lung histopathology.The expression of miR-34a in lung tissues and cells was detected by quantitative real-time PCR(qrt-PCR).Results the wet/dry weight of lung in the experimental group was significantly higher than that in the control group.Hematoxylin-eosin(HE)staining showed diffuse changes in lung tissue,thickening of alveolar septum,partial alveolar fusion and decreased number of alveoli in experimental group.The expression levels of miR-34a and TNF-α in the experimental group were higher than those in the control group at each time point.Conclusions It is speculated that miR-34a can promote the release of more inflammatory factors by positively regulating the expression of TNF-α,thus affecting the occurrence and development of lung injury.The expression of miR-34a positively correlated with TNF-α.miR-34a may affect the regulation mechanism of lung injury by mediating the expression of TNF-α through some signal pathway or inflammatory reaction,it provides a new therapeutic target for the treatment of neonatal acute respiratory distress syndrome(NARDS).
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