Therapeutic effect and mechanism of astragalus on acute respiratory distress syndrome in sepsis mice
Objective To verify the therapeutic effect of Astragalus on mice with acute respiratory distress syndrome with sepsis and to explore its mechanism.Methods Seventy SPF-grade C57 mice were divided into astragalus group(n=30),control group(n=30)and sham surgery group(n=10)according to random number table method,and CLP surgery was performed on Astragalus group and control group to induce sepsis acute respiratory distress syndrome,and CLP sham surgery was performed in the sham surgery group.After surgery,the astragalus group was treated with astragalus decoction for gastric gavage,the sham surgery group and the control group were gavaged with normal saline,and the mice were sacrificed 12 hours and 24 hours after the operation,and the lung histopathology was observed,the ratio of dry to wet weight of lung tissue,the protein concentration of alveolar lavage fluid was determined,the alveolar lavage fluid and serum were analyzed proteomics,and the differential proteins were enriched and analyzed.Results Astragalus reduced the total protein concentration of BALF in ARDS mice,reduced the dry-to-wet ratio of ARDS mice,and HE staining of lung tissues showed that Astragalus decoction improved acute alveolar injury in ARDS mice.Proteomic analysis of serum samples and BALF samples showed that there were certain differential proteins between astragalus group and control group,and enrichment analysis showed that it was mainly enriched in the pathway of inflammatory factors,confirming that astragalus decoction may play a role by inhibiting the activation and release of inflammatory factors.Conclusions Astragalus decoction can effectively reduce the inflammatory exudation of lung tissue in acute respiratory distress syndrome of sepsis,and its mechanism of action may be to inhibit the expression of inflammatory factors.
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