Mechanism of molecular hydrogen attenuating acute lung injury induced by lipopolysaccharid
Objective To investigate the role and mechanism of molecular hydrogen in lipopolysaccharide(LPS)-induced acute lung injury(ALI).Methods Balb/c male mice were randomly(random number)divided into control group,control+H2,LPS and LPS+H2 group with 6 mice in each group.The levels of malondialdehyde(MDA)and Fe2+in lung tissue were detected by kits.The lung tissue morphology was observed.The infiltration levels of F4/80 positive macrophages in lung tissue were detected by immunofluorescence staining.A549 cells were divided into control,control+H2,erastin and erastin+H2 group.The reactive oxygen species(ROS),malondialdehyde,(MDA),lactate dehydrogenase(GSH),number of cell death and lactate dehydrogenase(LDH)release in each group were detected by kits.Nrf2,GPX4,and HO-1mRNA were quantified by real-time PCR,the protein expression level of Nrf2 was detected by western blot,and the nuclear translocation level of Nrf2 was observed by immunofluorescence.The chi-square test was performed before the measurement data were counted.One-way analysis of variance was used to compare differences between multiple groups.Results Compared with the control group,the histopathological damage was aggravated,and the levels of MDA,Fe2+significantly increased in the LPS group,and F4/80 positive immune cells infiltration significantly increased(all P＜0.05).Compared with LPS group,the degree of lung injury in LPS+H2 group significantly reduced(all P＜0.05).In vitro experiments,compared with the control group,the ROS,MDA levels,number of cell death and LDH release significantly increased in erastin group(all P＜0.05),while GSH,and GPX4 mRNA levels decreased(all P＜0.05).HO-1mRNA and Nrf2 nuclear translocation levels increased(all P＜0.05).Compared with erastin group,ROS,MDA levels,cell death number and LDH release decreased in earstin+H2 group(all P＜0.05).The levels of GSH,GPX4 mRNA,Nrf2 mRNA,HO-1 mRNA and Nrf2 nuclear translocation levels increased(all P＜0.05).Conclusions Molecular hydrogen attenuates LPS-induced ALI by promoting Nrf2 nuclear translocation to inhibit ferroptosis of alveolar epithelial cells.
万方数据
维普
