核糖体调控因子1对人乳腺癌MDA-MB-468细胞增殖和转移能力的影响
Effect of regulator of ribosome synthesis 1 on proliferation and metastasis abilities of human breast cancer MDA-MB-468 cells
王润泽 1彭翠修 1宋军莹 1侯琳1
作者信息
- 1. 青岛大学基础医学院生物化学与分子生物学系,山东 青岛 266071
- 折叠
摘要
目的 探讨核糖体合成调控因子 1(RRS1)对人乳腺癌细胞增殖和转移能力的影响.方法 通过Western Blot实验检测人乳腺癌细胞系(MDA-MB-231、MDA-MB-468、BT549、MCF-7 细胞)以及正常人乳腺上皮细胞中 RRS1 蛋白的表达量;将 MDA-MB-468 细胞分别感染 sh-RRS1 慢病毒(sh-RRS1 组)和阴性对照慢病毒(Con组),未进行任何感染的 MDA-MB-468 细胞为Blank组,在荧光显微镜下观察 Con组和 sh-RRS1 组慢病毒感染效率,采用实时荧光定量PCR(RT-qPCR)技术和 Western Blot 实验分别检测各组细胞中RRS1 mRNA 和蛋白的表达水平;采用CCK-8 实验检测 RRS1 对 MDA-MB-468 细胞活力的影响;采用划痕实验、Transwell 实验以及侵袭实验检测 RRS1 对 MDA-MB-468 细胞侵袭和迁移能力的影响.结果 各乳腺癌细胞系中 RRS1 的表达水平均明显高于正常人乳腺上皮细胞(F=28.71,P＜0.05);相较于 Blank 组和 Con组,sh-RRS1 组细胞中RRS1 mRNA和蛋白的表达水平均显著降低(F=118.10、335.40,P＜0.05),细胞增殖活力明显减弱(F=825.60～2 839.00,P＜0.05),侵袭和迁移能力明显降低(F=25.60～430.80,P＜0.05).结论 RRS1 基因可能参与了乳腺癌细胞的增殖和转移过程,其作用可能与细胞中RRS1 的高表达有关.
Abstract
Objective To investigate the effect of regulator of ribosome synthesis 1(RRS1)on the proliferation and me-tastasis abilities of human breast cancer cells.Methods Western Blot was used to measure the protein expression level of RRS1 in human breast cancer cell lines(MDA-MB-231,MDA-MB-468,BT549,and MCF-7)and normal human breast epithelial cells.MDA-MB-468 cells were infected with sh-RRS1 lentivirus(sh-RRS1 group)and negative-control lentivirus(Con group),and MDA-MB-468 cells without infection were established as Blank group.Lentiviral infection efficiency was observed under a fluores-cence microscope for the Con group and the sh-RRS1 group,and quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of RRS1 in cells;CCK-8 assay was used to observe the effect of RRS1 on the viability of MDA-MB-468 cells;scratch assay,Transwell assay,and invasion assay were used to observe the effect of RRS1 on the invasion and migration abilities of MDA-MB-468 cells.Results The expression level of RRS1 in the breast cancer cell lines was signifi-cantly higher than that in normal human breast epithelial cells(F=28.71,P＜0.05).Compared with the Blank group and the Con group,the sh-RRS1 group had significant reductions in the mRNA and protein expression levels of RRS1(F=118.10,335.40,P＜0.05),cell proliferation activity(F=825.60-2839.00,P＜0.05),and the invasion and migration abilities of cells(F=25.60-430.80,P＜0.05).Conclusion The RRS1 gene might be involved in the proliferation and metastasis of breast cancer cells,which may be associated with the high expression of RRS1 in cells.
关键词
乳腺肿瘤/细胞系,肿瘤/核蛋白质类/核糖体调控因子1/细胞增殖/细胞运动Key words
Breast Neoplasms/Cell line,tumor/Nuclear proteins/Regulator of ribosome synthesis 1/Cell prolifera-tion/Cell movement引用本文复制引用
基金项目
国家自然科学基金资助项目(81472542)
山东省重点研发项目(2019GSF107025)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据