Effect of regulator of ribosome synthesis 1 on proliferation and metastasis abilities of human breast cancer MDA-MB-468 cells
Objective To investigate the effect of regulator of ribosome synthesis 1(RRS1)on the proliferation and me-tastasis abilities of human breast cancer cells.Methods Western Blot was used to measure the protein expression level of RRS1 in human breast cancer cell lines(MDA-MB-231,MDA-MB-468,BT549,and MCF-7)and normal human breast epithelial cells.MDA-MB-468 cells were infected with sh-RRS1 lentivirus(sh-RRS1 group)and negative-control lentivirus(Con group),and MDA-MB-468 cells without infection were established as Blank group.Lentiviral infection efficiency was observed under a fluores-cence microscope for the Con group and the sh-RRS1 group,and quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of RRS1 in cells;CCK-8 assay was used to observe the effect of RRS1 on the viability of MDA-MB-468 cells;scratch assay,Transwell assay,and invasion assay were used to observe the effect of RRS1 on the invasion and migration abilities of MDA-MB-468 cells.Results The expression level of RRS1 in the breast cancer cell lines was signifi-cantly higher than that in normal human breast epithelial cells(F=28.71,P＜0.05).Compared with the Blank group and the Con group,the sh-RRS1 group had significant reductions in the mRNA and protein expression levels of RRS1(F=118.10,335.40,P＜0.05),cell proliferation activity(F=825.60-2839.00,P＜0.05),and the invasion and migration abilities of cells(F=25.60-430.80,P＜0.05).Conclusion The RRS1 gene might be involved in the proliferation and metastasis of breast cancer cells,which may be associated with the high expression of RRS1 in cells.
Breast NeoplasmsCell line,tumorNuclear proteinsRegulator of ribosome synthesis 1Cell prolifera-tionCell movement
国家科技期刊平台
NSTL
万方数据
维普
