乳头状甲状腺癌组织中SPCS2基因表达水平及其意义
Expression and significance of the signal peptidase complex subunit 2 gene in papillary thyroid carci-noma
陈澎涛 1项承2
作者信息
- 1. 周口市中心医院甲状腺乳腺外科,河南周口 466000
- 2. 浙江大学医学院附属第二医院甲状腺外科
- 折叠
摘要
目的 探讨信号肽酶复合体亚基2(SPCS2)基因在乳头状甲状腺癌(papillary thyroid carcinoma,PTC)组织中的表达水平及其意义.方法 利用UALCAN及HPA数据分析平台评估PTC组织和正常组织中SPCS2基因表达水平.在Gepia数据库预测SPCS2基因表达水平对PTC患者无病生存时间的影响.Cox回归模型分析影响PTC患者预后的独立危险因素.并对该基因进行KEGG、GO和GSEA分析,确定参与PTC的重要信号通路.结果 PTC患者肿瘤组织中SPCS2基因表达水平均显著低于正常组织(Z=-43.219,P＜0.001).与SPCS2低表达的PTC患者相比,SPCS2基因高表达组PTC患者无病生存时间更长(P＜0.05).Cox回归模型分析显示,PTC患者肿瘤组织中SPCS2低表达是导致PTC患者预后不良的独立危险因素(HR=0.573,P＜0.05)o富集分析预测显示,线粒体氧化磷酸化通路富集在SPCS2高表达组.结论 PTC患者肿瘤组织中SPCS2基因表达水平下调,SPCS2基因可能参与线粒体氧化磷酸化信号通路的调控,该基因可能是评估PTC患者预后的重要生物标志物.
Abstract
Objective To investigate the expression level and significance of the signal peptidase complex subunit 2(SPCS2)gene in papillary thyroid carcinoma(PTC).Methods UALCAN and HPA Databases were used to investigate the mRNA expression levels of SPCS2 in PTC tissue and normal tissue.Gepia Database was used to predict the influence of the expres-sion level of SPCS2 on the disease-free survival of PTC patients.The Cox regression model was used to investigate the independent risk factors for the prognosis of PTC patients.GO,KEGG,and GSEA analyses were performed for SPCS2 to identify the impor-tant signaling pathways involved in PTC.Results The mRNA expression levels of SPCS2 in PTC tissue were significantly lo-wer than those in normal tissue(Z=-43.219,P＜0.001).The PTC patients with a high expression level of SPCS2 had a longer disease-free survival time than those with a low expression level of SPCS2(P＜0.05).The Cox regression analysis showed that the low expression level SPCS2 in PTC patients was an independent risk factor for the poor prognosis of PTC patients(HR=0.573,P＜0.05).The enrichment analysis showed that the oxidative phosphorylation pathway of mitochondria was enriched in the SPCS2 high expression group.Conclusion The expression level of SPCS2 is downregulated in PTC patients,and SPCS2 may partici-pate in the regulation of the oxidative phosphorylation pathway of mitochondria.SPCS2 may be an important biomarker for evalua-ting the prognosis of PTC patients.
关键词
甲状腺癌,乳头状/基因表达/SPCS2基因/预后/线粒体/氧化磷酸化/代谢网络和途径Key words
Thyroid cancer,papillary/Gene expression/SPCS2 gene/Prognosis/Mitochondria/Oxidative phosphory-lation/Metabolic networks and pathways引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据