首页|血小板衍生生长因子对脉络膜黑色素瘤细胞活性及侵袭能力的影响

血小板衍生生长因子对脉络膜黑色素瘤细胞活性及侵袭能力的影响

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的 探讨血小板衍生生长因子(PDGF)对脉络膜黑色素瘤(choroidal melanoma，CM)细胞活性及侵袭能力的影响。方法 将CM细胞分为A、B组，分别转染空载体和敲降PDGF的慢病毒，采用RT-qPCR技术检测两组细胞中PDGF mRNA相对表达量，采用XTT实验检测两组细胞活性，采用Transwell实验检测两组细胞侵袭能力，采用Western blot实验检测两组细胞上皮-间质转化(EMT)及基质金属蛋白酶(MMPs)相关标志物蛋白的相对表达量。GEPIA数据库分析PDGF水平与CM患者预后的关系。结果 与A组比较，B组细胞中PDGF RNA相对表达量下降(t=176。30，P＜0。05)，细胞存活数目下降及侵袭能力减弱(F=57。21，t=14。10，P＜0。05)。W estern blot实验结果显示，与A组相比，B组细胞中E-cadherin相对表达量升高，N-cadherin、Snail、Vimentin、MMP9 及 MT1 MMP 的相对表达量下降(t=4。13～14。14，P＜0。05)。GEPIA 数据库分析显示，PDGF-A、PDGF-B高表达与CM患者的不良预后有关(P＜0。05)。结论 PDGF具有增强CM细胞活性，促进CM细胞侵袭的作用，其机制可能与诱导细胞EMT的发生有关。
Effect of platelet-derived growth factor on the activity and invasion ability of choroidal melanoma cells
Objective To investigate the effect of platelet-derived growth factor(PDGF)on the activity and invasion ability of choroidal melanoma(CM)cells.Methods CM cells were divided into groups A and B and were transfected with empty vector and PDGF knockdown lentivirus,respectively.RT-qPCR was used to measure the relative expression level of PDGF mR-NA;XTT assay was used to measure cell activity;Transwell assay was used to test the invasion ability of cells in the two groups;Western blot was used to measure the relative expression levels of epithelial-mesenchymal transition(EMT)marker proteins and matrix metalloproteinases(MMPs).GEPIA database was used to investigate the association between PDGF and the prognosis of CM patients.Results Compared with group A,group B had significant reductions in the relative expression level of PDGF RNA(t=176.30,P＜0.05),the number of cells which survived(F=57.21,P＜0.05),and the invasion ability of cells(F=57.21,t=14.10,P＜0.05).Western blot showed that compared with group A,group B had a significant increase in the relative ex-pression level of E-cadherin and significant reductions in the relative expression levels of N-cadherin,Snail,Vimentin,MMP9,and MT1 MMP(t=4.13-14.14,P＜0.05).The GEPIA database analysis showed that the high expression levels of PDGF-A and PDGF-B were associated with the poor prognosis of CM patients(P＜0.05).Conclusion PDGF can enhance the activity of CM cells and promote the invasion of CM cells,possibly by inducing EMT.

MelanomaChoroid neoplasmsPlatelet-derived growth factorCell proliferationNeoplasm invasivenessEpithelial-mesenchymal transitionMatrix metalloproteinases

姚宁宁、袁莹莹、马清悦、易雯丹、隋爱华、罗文娟

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青岛大学附属医院眼科,山东青岛 266003

青岛大学附属医院医学研究中心,山东青岛 266003

黑色素瘤 脉络膜肿瘤 血小板源性生长因子 细胞增殖 肿瘤浸润 上皮-间质转化 基质金属蛋白酶类

山东省自然科学基金面上项目

82274585

2024

10.13362/j.jpmed.202404008

精准医学杂志
青岛大学

精准医学杂志

ISSN：2096-529X
年,卷(期)：2024.39(4)