Expression and significance of Ninj1,IL-1β and PGP-9.5 in ectopic endometrial tissues of patients with endometriosis
Objective To investigate the expression levels of nerve injury-induced protein 1(Ninj1),interleukin-1β(IL-1β),and protein gene product 9.5(PGP-9.5)in the ectopic endometrial tissues of patients with endometriosis(EMT)and their re-lationship with dysmenorrhea.Methods Ectopic endometrial tissues from 40 patients with ovarian EMT diagnosed by post-ope-rative gynecological pathological examination in our hospital from April 2020 to April 2021 were selected as group A.In situ endo-metrial tissues from 20 of these patients were obtained as group B.Normal endometrial tissues from 20 patients with uterine fi-broids in the same period were collected as group C.Group A was divided into a dysmenorrhoea subgroup and a non-dysmenorrhoea subgroup according to the presence or absence of dysmenorrhoea,with 20 patients per subgroup.The expression levels of Ninj1,IL-1β,and PGP-9.5 in the endometrial tissues of groups A,B,and C were measured by immunohistochemistry,and their correla-tion was analyzed.The expression level of IL-1β in the peripheral blood of patiens in groups A and C was measured by enzyme-linked immunosorbent assay.Results The expression level of IL-1β in the peripheral blood of patients in group A was signifi-cantly higher than that in group C(t=-4.184,P＜0.05).The positive expression rates of three proteins in the endometrial tissues were significantly increased in groups A and B compared with group C(x2=4.286-19.394,P＜0.05)and in group A compared with group B(x2=4.149-5.272,P＜0.05).The positive expression rates of Ninj1 and PGP-9.5 proteins in the endometrial tissues were significantly higher in the dysmenorrhoea subgroupthan in the non-dysmenorrhoea subgroup(x2=8.533,11.905,P＜0.05).The expression of Ninj1 was positively correlated with the expression of PGP-9.5 and IL-1β in the endometrial tissues of group A(r=0.733,0.628,P＜0.05).Conclusion Ninj1,IL-1β,and PGP-9.5 are highly expressed in the ectopic endometrial tissues,which may be involved in the occurrence and development of EMT.The positive expression rates of both Ninj1 and PGP-9.5 in the endometrial tissues of the dysmenorrhoea subgroup were higher than those in the non-dysmenorrhoea subgroup,which may be closely related to the mechanism of EMT pain.
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