Sex determination of pupae and adults of Diaphania caesalis ( Walker)
A rapid, simple and accurate method for identifying the sex of Diaphania caesalis ( Walker ) pupae and adults was described. Some significant differences between male and female were found by observing abdominal morphological characteristics of Diaphania caesalis ( Walker ) pupae and adults. The results showed that female pupae have a lengthways suture on the 8 th abdominal segment, which is composed of genital pore and oviporus. The distance between the genital pore and anal opening in female pupae is greater than in male pupae. However, the genital pore in male pupae is located on the 9 th abdominal segment with two distinct semi-circular strumae. But the section of the last abdominal segment not obvious compared to female pupae. The sex of adults can be determined based on the color and length of tail hair at the end of abdomen. Male have the long black tail hair and female do not. This rapid assessment method is efficient for use in the field and has the potential to vastly improve determining the sex ratio, predicting population trends, artificial feeding indoor and describing biological properties in Diaphania caesalis ( Walker) .