Functional and metagenomic applications of black soldier fly larvae gut microbiota,Hermetia illucens(Diptera:Strationyidae)
Many kinds of microbes inhabit the gut of black soldier fly larvae,and the interactions between these microbes and the host are extremely complex.They can affect the host's growth,reproduction,nutrition metabolism,behavior preference and lifespan.In addition,they can also regulate the host's immune system and protect the host from pathogen invasion.Understanding the mechanisms of these interactions is crucial for artificial mass breeding of black soldier fly.Although some articles have summarized the mechanisms of microbe-black soldier fly interactions,most studies aiming to characterize the functions of insect gut microbiota are based on high-throughput sequencing of 16S rRNA gene,which can only provide structural information of relevant microbial communities,but cannot reveal the functions and metabolic capacities of microbes.Therefore,some functional methods such as metagenomics,metatranscriptomics,metaproteomics and metabolomics are also gradually applied.These techniques not only provide a complete classification of microbial populations,but also reveal their functions and metabolic capacities.By understanding the composition and function of gut microbiota,the feed,breeding environment and growth conditions of black soldier fly can be effectively adjusted,thereby enhancing the stability of black soldier fly gut microbiota,improving the production performance and economic benefits of black soldier fly.
Black soldier flygut microbiotafunctionmetagenomic