Study on the calling and mating rhythm of Tuta absoluta
Understanding the rhythms of insect reproductive behaviors,such as calling and mating,could provide instructions for the development and application of sex pheromone attractants.In this study,the courtship behavior rhythm of females and mating of male and female adults in Tuta absoluta were explored.The results showed that,except for a small proportion of females called before the photophase,the calling behavior of the majority of female adults was observed in the photophase,with the calling rate increased rapidly at the beginning of the photophase,and reached the peak at more than 80%within 0.5 h.The calling rate of females at different ages was different,with 2 to 3-day-old females the most active.The courtship behavior mainly occurred in the photophase,with the peak mating rate over 90%at 0.5 h in the photophase.Both females and males of T.absoluta could mate multiple times,and the highest mating frequency of females was 4 times in 7 days.Compared with the male-female ratio of 1∶1,the mating rate in 1∶2 female-male ratio treatment slightly decreased,while the mating rate and average mating duration in 2∶1 female-male ratio treatment increased.This study confirmed that the calling and mating of T.absoluta were mainly in the photophase,and there was obvious rhythm.Both female and male of T.absoluta can mate multiple times.