Diversity analysis of midgut bacteria community of tomato leaf miner Tuta absoluta(Meyrick)in Hexi arid region
In order to analyze the diversity and relative abundance of intestinal endosymbiotic bacteria in different geographic populations of tomato leaf miner moth,this study analyzed the diversity and relative abundance of intestinal endosymbiotic bacteria in tomato leaf miner moth by 16S rDNA high-throughput sequencing,and analyzed the differences in gene metabolic pathways of endosymbiotic bacterial biomes by KEGG.The results showed that the Jiuquan population was relatively higher than Zhangye in terms of abundance,diversity,evenness and evolutionary diversity.The top 20 midgut bacteria in the abundance of the two geographic populations belonged to Actinomyceta,Bacteroides,Firmicutes,Proteobacterium and Verrucomicrobia,respectively,of which the relative abundance of Firmicutes and Proteobacteria bacteria were all higher.The highest relative abundance of Zhangye geographic population was Wolbachia(16.06％),which belongs to Proteobacteria;the highest relative abundance of Jiuquan geographic population was Lactobacillus(13.75％),which belongs to Firmicutes;and the largest difference in relative abundance between two geographic populations was in the Wolbachia genus,with the relative abundance of the Zhangye geographic population being 18.89 times higher than that of the Jiuquan geographic population.In addition,33 gene functions of microbial communities were predicted in this study,and the main community gene functions were metabolic functions,among which carbohydrate metabolism was the strongest,and there was no significant difference between populations;the most significant differences in metabolic functions between two geographic populations were xenobiotics biodegradation and metabolism,with Jiuquan populations being relatively stronger.Overall,the diversity of bacteria in the midgut of the two geographical populations was small,among the species,Stenotrophomonas might be closely related to the resistance of pests,and the bacteria of Wolbachia could be applied to biological control of pests with a good prospect.
Tuta absolutabacteria communitycommunity differencesgeographic populationmetabolic pathway
万方数据
维普
