The effect of RNA interference of Cflac1 gene on the response of termite intestinal redox potential to pathogenic infection
Coptotermes formosanus Shiraki,a wood-feeding subterranean termite,is one of a globally distributed economic pests.Biocontrol of subterranean termites is largely impeded by their immune defenses.The detoxifying enzymes of phytophagous insects play a crucial role in their digestion and immune regulation.The study on the regulation of redox homeostasis by cletoxifying enzymes with lignin metabolism activity in the intestinal immune process of termites is of great significance for revealing the intestinal immune mechanism of termites.Here,Metarhizium anisopliae was using as an immune inducer.The variations in redox potential in the foregut/salivary gland,midgut,and hindgut of pathogen infected termites was firstly analyzed by using a microelectrode detection system.By using RNA interference(RNAi)technology,the expression of the laccase gene Cfflacl,which was involved in lignin metabolism activity,was inhibited in the infected/uninfected termites.And the importance of Cfflacl in maintaining redox homeostasis in the intestinal immune defense against fungi in C.formosanus was also determined.This study enriches the research content on the intestinal immune mechanism of termites,providing theoretical basis for expanding new targets for termite control and the development of biological control technologies.
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